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You Searched For: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.


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Product Image-CA89131-002

β-Galactosidase Assay, G-Biosciences

Catalog Number: (CA89131-002)
Supplier: G-Biosciences
Description: G-Biosciences' β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct.

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Product Image-89156-478

Matrix Metalloproteinase-8 (MMP-8) Colorimetric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-478)
Supplier: Enzo Life Sciences
Description: A QuantiZymeTM Assay System
Product Image-89141-836

Akt kinase activity kit, Enzo Life Sciences

Catalog Number: (89141-836)
Supplier: Enzo Life Sciences
Description: For the measurement of Akt in partially purified, purified, or crude enzyme preparations from any species.
Product Image-89156-484

Matrix Metalloproteinase-14 (MMP-14) Fluorometric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-484)
Supplier: Enzo Life Sciences
Description: A QuantiZymeTM Assay System
Product Image-89156-514

FLUOR DE LYS® HDAC8 Fluorometric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-514)
Supplier: Enzo Life Sciences
Description: A Fluor de Lys® fluorescent assay system
Product Image-89156-494

Cathepsin S Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-494)
Supplier: Enzo Life Sciences
Description: A QuantiZyme™ Assay System
Product Image-89156-462

Matrix Metalloproteinase-7 (MMP-7) Colorimetric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-462)
Supplier: Enzo Life Sciences
Description: A QuantiZymeTM Assay SystemThe MMP-7 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a thiopeptide as a chromogenic substrate.
Product Image-89156-460

Matrix Metalloproteinase-1 (MMP-1) Fluorometric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-460)
Supplier: Enzo Life Sciences
Description: A QuantiZymeTM Assay System
Product Image-89156-468

Matrix Metalloproteinase-2 (MMP-2) Fluorometric Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-468)
Supplier: Enzo Life Sciences
Description: A QuantizymeTM Assay System
Product Image-89156-496

Neutrophil elastase colorimetric drug discovery kit, Enzo Life Sciences

Catalog Number: (89156-496)
Supplier: Enzo Life Sciences
Description: It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.
Product Image-89156-418

MMP Inhibitor Profiling Kit, Fluorometric, Enzo Life Sciences

Catalog Number: (89156-418)
Supplier: Enzo Life Sciences
Description: A QUANTIZYME® Assay System Complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes. The assays are performed in a convenient 96-well microplate format using a sensitive fluorogenic substrate . Included are active MMP enzymes, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet.
Product Image-89156-440

CV-Cathepsin B Detection Kit, Enzo Life Sciences

Catalog Number: (89156-440)
Supplier: Enzo Life Sciences
Description: Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.
Product Image-89156-492

Cathepsin K Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-492)
Supplier: Enzo Life Sciences
Description: The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit.
Product Image-89156-558

PTP1B Drug Discovery Kit, Enzo Life Sciences

Catalog Number: (89156-558)
Supplier: Enzo Life Sciences
Description: The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.
Product Image-89156-526

FLUOR DE LYS® SIRT3 Fluorometric Drug Discovery Assay Kit, Enzo Life Sciences

Catalog Number: (89156-526)
Supplier: Enzo Life Sciences
Description: A FLUOR DE LYS® fluorescent assay system
Product Image-89156-424

BIOMOL® Green, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: BIOMOL Green™ provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL Green™ doesn’t require multiple solutions or reagents prepared fresh on the day of the assay. BIOMOL Green™ is extremely stable (>6 mos. at 4°C) and is simply mixed, at room temperature, with any enzymatic reaction that has released free phosphate. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.BIOMOL Green™ has perhaps been most widely applied to protein phosphatase assays. Typically, a protein phosphatase (e.g. PTP1B or LAR) is simply incubated with an appropriate phosphopeptide (e.g. IR5,9,10). Addition of BIOMOL Green™ stops the reaction and begins color development, which is read 20-30 min. later.BIOMOL Green™ may be used in cuvette or microplate-based assays and is ideal for high-throughput applications. Each kit comes with a standardized phosphate solution for assay calibration.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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