EnzyChrom™ Fumarase Assay Kit, BioAssay Systems

Supplier: BioAssay Systems

EnzyChrom™ Fumarase Assay Kit, BioAssay Systems

EnzyChrom™ Fumarase Assay Kit, BioAssay Systems

EnzyChrom™ Fumarase Assay Kit, BioAssay Systems

EnzyChrom™ Fumarase Assay Kit, BioAssay Systems

EFMR-100

75878-090EA 1221.8 CAD

75878-090

EnzyChrom™ Fumarase Assay Kit, BioAssay Systems

Assays Enzyme Assays

For quantitative determination of fumarase enzyme activity.

Linear detection range 0.4 to 70 U/L
Homogeneous "mix-incubate-measure" type high-throughput assay

Fumarase (or fumarate hydratase) (EC 4.2.1.2) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate

BioAssay Systems’ non-radioactive, colorimetric fumarase assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc.

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