QuantiChrom™ Glucosidase Assay Kit, BioAssay Systems

Supplier: BioAssay Systems
DBGD-100
75877-964EA 1083.26 CAD
75877-964
QuantiChrom™ Glucosidase Assay Kit, BioAssay Systems
Assays Enzyme Assays
For quantitative determination of beta-glucosidase activity and evaluation of drug effects on its metabolism.

  • Linear detection range 2 to 250 U/L
  • Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes
  • Can be readily automated on high-throughput liquid handling instruments

β-Glucosidase is a glucosidase enzyme which acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose)

BioAssay Systems’ QuantiChrom™ β-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

β-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a β-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a β-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher’s disease and Parkinson’s disease. Simple, direct and automation-ready. Procedures for measuring β-glucosidase activity are becoming popular in Research and Drug Discovery.
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