Nitric Oxide (total) Assay Kit, Multispecies, Thermo Scientific

Supplier: Thermo Scientific
EMSNOTOT
CAPIEMSNOTOTEA 4152.6 CAD
CAPIEMSNOTOT
Nitric Oxide (total) Assay Kit, Multispecies, Thermo Scientific
Assays Cellular Assays
Assay nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Nitric Oxide Assay Kit.

Features of the Nitric Oxide Assay Kit:

Target: total nitric oxide (NO) contributed by the sum of nitrite (NO2-) and nitrate (NO3-)
Format: colorimetric stain (azo dye) for clear 96-well plates
Measurement: absorbance at 540nm (±20nm)
Assay Range: 3.1 to 100µM
Sensitivity:1.35µM
Precision: dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)
Sample Types: urine, saliva, plasma, serum and other biological fluids
Total Assay Time: 45 minutes
Sample Size: 50 µL

This nitric oxide assay kit is for quantitative determination of nitrite (NO2-) and nitrate (NO3-) in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540nm. Total nitric oxide (NO) contributed by nitrate and nitrite in a system is measured as nitrite after converting all nitrate to nitrite.

Includes:
Complete kit contains Griess reagents, nitrite and nitrate standards, nitrate reductase, NADH, and diluents.

Ordering information: For Research Use Only. Not for use in diagnostic procedures. Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8˚C. See product manual for detailed contents and storage conditions for maximum stability.
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