Assay nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Nitric Oxide Assay Kit.
Features of the Nitric Oxide Assay Kit:
Target: total nitric oxide (NO) contributed by the sum of nitrite (NO2-) and nitrate (NO3-)
Format: colorimetric stain (azo dye) for clear 96-well plates
Measurement: absorbance at 540nm (±20nm)
Assay Range: 3.1 to 100µM
Sensitivity:1.35µM
Precision: dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)
Sample Types: urine, saliva, plasma, serum and other biological fluids
Total Assay Time: 45 minutes
Sample Size: 50 µL
This nitric oxide assay kit is for quantitative determination of nitrite (NO2-) and nitrate (NO3-) in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540nm. Total nitric oxide (NO) contributed by nitrate and nitrite in a system is measured as nitrite after converting all nitrate to nitrite.
Includes:
Complete kit contains Griess reagents, nitrite and nitrate standards, nitrate reductase, NADH, and diluents.
Ordering information: For Research Use Only. Not for use in diagnostic procedures. Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8˚C. See product manual for detailed contents and storage conditions for maximum stability.