Pierce™ DAB Substrate Kit, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ DAB Substrate Kit, Thermo Scientific

Pierce™ DAB Substrate Kit, Thermo Scientific

Pierce

34002 34002

CAPI34002EA 327.31 CAD

PI34002 CAPI34002

Pierce™ DAB Substrate Kit, Thermo Scientific

Electrophoresis Reagents Western Blotting Reagents

The Thermo Scientific Pierce DAB Substrate Kit enables chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in Western blot and tissue staining methods.

Easy-to-use—dilute 10X stock solution to 1X in provided stable peroxide buffer
Safer—preformulated DAB solution minimizes safety concerns with this chemical
Complete—includes convenient solution of stable hydrogen peroxide buffer
Store kit at 4˚C.

DAB (3,3'-diaminobenzidine tetrahydrochloride; MW = 214.1) reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples. The brown precipitate is insoluble in alcohol and other organic solvents, making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media. The Pierce DAB Substrate Kit includes a 10X solution of DAB and Stable Peroxide Substrate Buffer. Crystalline DAB (powder) is also available.

Delivery information: Kit contains DAB Solution (10X), 25 ml; Stable Peroxide Substrate Buffer, 250 ml.

Caution: For Research Use Only. Not for use in diagnostic procedures.

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