Nucleic acid labelling and detection system, AlkPhos Direct™

Supplier: Cytiva
RPN3682 RPN3685 RPN3682 RPN3688 RPN3680 RPN3680 RPN3685 RPN3688
CA95038-288LEA 2042.24 CAD
95038-292 CA95038-288L CA95038-298L CA95038-296L 95038-288 95038-298 95038-296 CA95038-292L
Nucleic acid labelling and detection system, AlkPhos Direct™
Electrophoresis Reagents Western Blotting Reagents
AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

  • Fast and convenient system for all high sensitivity applications using DNA, RNA, or oligonucleotide probes
  • Hybridisation stringency can be controlled by elevating temperatures up to 75 °C
  • Detection of 60 fg target DNA (single-copy gene) in a genomic southern blot
  • ECF detection for quantitation experiments
  • Saves 2 to 3 hours over conventional indirect methods

Labelling is complete within 30 minutes in a single tube protocol and the resulting probe is ready for use in hybridisations without further purification. Due to the thermostable nature of the enzyme, hybridisation stringency can be controlled by adjusting temperature as well as salt concentration. The system can be used with either chemiluminescent or fluorescent detection reagents. The most sensitive result is achieved with CDP-Star™, a chemiluminescent substrate with a fast light output. Alternatively, the use of ECF substrate generates a fluorescent signal suitable for use with fluorescence scanning devices.

Delivery information: Each system includes labelling reagent, crosslinker, control DNA, reaction buffer, hybridisation buffer, blocking agent, CDP-Star™ or ECF detection reagents.
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