Amersham ECL Direct Labeling and Detection System, Cytiva

Supplier: Cytiva
ECL™
RPN3001 RPN3001 RPN3000 RPN3000
CA95038-272LEA 4854.35 CAD
95038-270 CA95038-272L 95038-272 CA95038-270L
Amersham ECL Direct Labeling and Detection System, Cytiva
Electrophoresis Reagents Western Blotting Reagents
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

  • Direct probe labelling in a 10 minute reaction, 1 hour from hybridization to detection with ECL Direct and Hybond™ N+.
  • Eliminates handling, waste and regulatory issues associated with use of radioactivity.
  • No need to strip blots before reprobing.
  • Contains a novel rate enhancer, giving higher sensitivity with very low backgrounds on nylon membranes.

Delivery information: ECL Direct is particularly useful for applications that require a blot to be reprobed several times and also for screening applications requiring rapid throughput and fast results. Examples including High-to-Medium-target Southern and Northen blots, Colony/ Plaque lifts, Dot or Slot Blots, Genome Mapping, PCR Product Analysis, Library Screens.
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