Calcein AM ≥95% (by HPLC), Ultrapure

Supplier: Enzo Life Sciences

Calcein AM ≥95% (by HPLC), Ultrapure

Calcein AM ≥95% (by HPLC), Ultrapure

Synonyms: Calcein-AM , Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester , Calcein acetoxymethyl ester , Tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)bis(methylene))bis(azanetriyl))tetraacetate

ENZ-52002

89165-934EA 279 CAD

89165-934

Calcein AM ≥95% (by HPLC), Ultrapure

Calcein AM

Calcium dye

Calcein acetomethoxy (AM), a derivative of calcein, is useful for differentiating between live and dead cells. Calcein AM readily passes through the cellular plasma membrane. Once inside, esterases cleave the AM groups yielding the more hydrophilic calcein, trapped inside the cell. The loss of the acetomethoxy group also enables calcein to readily bind intracellular calcium; resulting in a strong yellowish-green fluorescence. As dead cells lack cytoplasmic esterases, fluorescence is demonstrated exclusively in live cells, making the probe useful for determining cell viability. Calcein-AM exhibits low cytotoxicity and does not significantly affect cellular functions such as proliferation or chemotaxis. Viability assays using calcein correlate well with other assays, such as 51Cr-release. Wavelength Maxima: Excitation 495nm, Emission 515nm

Formula: C₄₆H₄₆N₂O₂₃
MW: 994.86 g/mol
Storage Temperature: Freezer

MDL Number: MFCD05861516
CAS Number: 148504-34-1

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