ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.
Micromolar concentrations of ER-ID® Red dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line, human T-lymphocyte cell line, Jurkat, HeLa and human bone osteosarcoma epithelial cell line, U2OS
The ER-ID® Red assay kit GFP-CERTIFIED®) is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as fluorescein, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.
One important application of ER-ID® Red dye is in fluorescence co-localization imaging with green fluorescent protein (GFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.
Delivery information: Kit includes ER-ID® Red detection reagent (50 μL), Hoechst 33342 nuclear stain (50 μL), 10X Assay buffer (15 mL).
