The ER-ID® Green assay kit is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs).
- Easily multiplexed with common fluorescent dyes and fluorescent proteins
- Highly resistant to photo-bleaching, concentration quenching and photo-conversion
- Stringently manufactured, to control and eliminate non-specific assay artifacts
ER-ID™ Green assay kit contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. Micromolar concentrations of ER-ID™ Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line, human T-lymphocyte cell line, Jurkat, HeLa and human bone osteosarcoma epithelial cell line, U2OS.One important application of ER-ID™ Green dye is in fluorescence co-localization imaging with red fluorescent protein (RFP)- or orange fluorescent protein (OFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.
Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.
Delivery information: Kit includes ER-ID® Green detection reagent (50 µL), Hoechst 33342 nuclear stain (50 µL), 10X Assay buffer (15 mL).
