LYSO-ID® Red Cytotoxicity Kit (GFP-CERTIFIED®), Enzo Life Sciences

Supplier: Enzo Life Sciences

51015-KP002

89165-892EA 677.99 CAD

89165-892

LYSO-ID® Red Cytotoxicity Kit (GFP-CERTIFIED®), Enzo Life Sciences

Assays Cellular Assays

A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay

96-well cell-based assay. Provides a rapid and quantitative approach for determining drug- or toxic agent-induced lysosome and lysosome-like organelle perturbations in live cells.

The Lyso-ID® Red Cytotoxicity Kit monitors dysfunction of lysosomal degradation using a drug-like cationic amphiphilic tracer (CAT) dye that rapidly and selectively stains acidic organelles, and is suitable for monitoring accumulation of lysosomes and lysosome-like structures in live cells.

Mechanism of Action
A cationic amphiphilic tracer (CAT) dye that rapidly partitions into cells in a similar manner as drugs that induce phospholipidosis. This tracer was created by placing titratable groups on the probe (dye), to enable labeling to be expanded into lamellar inclusion bodies of cells pretreated with weakly basic cell-permeant compounds, such as the antimalarial drug chloroquine. Besides lysosomes themselves, the probe can be employed for highlighting lysosome-like organelles under conditions wherein cells produce vacuoles that contain most of the degradative enzymes of the lysosome but are not as acidic as the parent organelle.

Ordering information: Quantity: 2 x 96-well plates Contents: 10x dual color detection reagent: 2 x 1ml
Detection buffer: 20ml
Verapamil control: 3μmoles
10x assay buffer: 15ml

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