Bio-16-UTP, Enzo Life Sciences

Supplier: Enzo Life Sciences

ENZ-42814

89165-832EA 512 CAD

89165-832

Bio-16-UTP, Enzo Life Sciences

Nucleic Acid Reagents Nucleotides

Bio-16-UTP (Biotin-16-uridine-5’-triphosphate) can replace UTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

Appearance: Clear, colorless liquid
Purity: ≥93% (HPLC)
Concentration: 10 mM
Extinction coefficient: 10,700 M^-1 cm^-1 (240 nm, pH 7,0)

The biotin-labelled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridisation, sequencing, and genome analysis.

The biotin-labelled hybridised probes can be detected by a reporter molecule linked to streptavidin, avidin, or anit-biotin antibody. Such a complex can be detected directly, e.g. by excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble coloured precipitate.

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