Bio-16-dUTP (Biotin-16-2’-deoxyuridine-5’-triphosphate) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
Bio-16-dUTP can be used to produce biotinylated DNA probes in a variety of labelling reactions including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. The resulting biotin-labeled probe can be used in a variety of hybridization applications incuding Southern blots, Northern blots, or dot blots. The probes can also be used for in situ hybridization procedures on fixed cells and tissues. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by their interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color-producing enzymes.
