Auto-Ubiquitinylation Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

Auto-ubiquitinylation kit

Auto-ubiquitinylation kit

UW0970-0001

89165-248EA 506 CAD

89165-248

Auto-Ubiquitinylation Kit, Enzo Life Sciences

Assays Protein Assays

This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation

Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.

Delivery information: Kit contains 20X Ubiquitin Activating Enzyme Solution (E1) (25µL), 20X Ubiquitin Conjugating Enzyme Solution (E2) (25µL), 10X Ubiquitin Solution (Ub) (25µL), 20X Control Ubiquitin Ligase Solution (E3) (25µL), 20X Mg-ATP Solution (25µL), 10X Ub E3 ligase Buffer (50µL), Ubiquitin antibody solution (25µL).

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