ApoSENSOR™ ADP/ATP Ratio Assay Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

ApoSENSOR™ ADP/ATP Ratio Assay Kit, Enzo Life Sciences

ApoSENSOR™ ADP/ATP Ratio Assay Kit, Enzo Life Sciences

850-248-KI01

89156-018EA 1370 CAD

89156-018

ApoSENSOR™ ADP/ATP Ratio Assay Kit, Enzo Life Sciences

Assays Cellular Assays

The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.

The assay utilizes bioluminescent detection of ADP and ATP levels for rapid screening of apoptosis (TUNEL-based techniques and caspase assays), necrosis, growth arrest and cell proliferation simultaneously in mammalian cells

This highly sensitive assay (detects 100 mammalian cells/well) can be fully automated for high throughput measurements.

The assay utilizes the enzyme luciferase to catalyze the formation of light fom ATP and luciferin. The light can be measured using a luminometer or beta counter. ADP levels are measured by its conversion to ATP that is subsequently detected using the same reaction.

Delivery information: Kit includes Nucleotide Releasing Buffer (50 mL), 1 vial ATP Monitoring Enzyme (green cap), (1 vial) ADP Monitoring Enzyme (blue cap), 450 µL Enzyme Reconstitution Buffer (red cap).

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