Phosphothreonine detection set, Enzo Life Sciences

Supplier: Enzo Life Sciences

850-024-KI01

89155-884EA 879 CAD

89155-884

Phosphothreonine detection set, Enzo Life Sciences

Protein Linking and Labeling Systems

This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites.

Immunoprecipitation; Western Blot (20µl/lane (mini gel) for HRPO/DAB detection, or 5µl/lane (mini gel) for HRPO/ECL detection). Do not use milk/casein for blocking and dilution! Replace by BSA! We recommend to use 1% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 3500, 0.2% Tween 20 in 2x PBS.Optimal conditions must be determined individually for each application.

Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.

Ordering information: Positive control included. Contents: Contains 25µg each of clones:1E11 (Prod. No. ALX-804-168)4D11 (Prod. No. ALX-804-169)14B3 (Prod. No. ALX-804-170)

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