You Searched For: Protein Purification

qPCR qPCR stands for Quantitative Real-Time Polymerase Chain Reaction and is a technique which monitors in real-time the enzymatic amplification of a targeted sequence of DNA. Analysis of the resultant amplification curves informs a researcher of ...

Downstream purification for cell therapy Comparing downstream purification approaches between cell therapies and other biologics is quite different. Methods required to obtain the final cell dose with the required quality parameters include: Centr...

33 LC Colum n Selection – C18 Reversed-Phase M aterials www.hichrom.co.uk • sales@hichrom.co.uk • Tel: +44 (0) 118 930 3660 Hichrom Lim ited C18 Reversed-Phase Materials SPECIFICATIONS OF C18 BONDED RP MATERIALS Octadecyl (ODS) or C18 bonded phase...

TECHNICAL BULLETIN “Pipetting error” is a phrase that is commonly heard in research laboratories. When preparing reagent cocktails pipetting error is accommodated for. What is not typically discussed or published are the causes and ramifications o...

Nucleic Acid Purification"Nucleic Acid Purification

Downstream purification for vaccines During subsequent steps for vaccine concentration and clarification followed by passage through several filtration steps and chromatography columns it is critical that vaccine purity potency and consistency be ...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

Downstream purification for vaccines During subsequent steps for vaccine concentration and clarification followed by passage through several filtration steps and chromatography columns it is critical that vaccine purity potency and consistency be ...

Click to edit Master title style Got Protein? Testing the protein content of common foods Bradford Protein Assay Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller...

Introduction to SDS-PAGE – Separating Proteins Based on Molecular Weight Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis or SDS-PAGE is a widely used technique for separating proteins based on their molecular weight. In this technique pr...

What is western blotting? Overview of western blotting Western blotting is the technique for identifying proteins of interest in a sample using gel electrophoresis to separate sample proteins. This technique has paved the way in biomedical science...

PowerPoint Presentation Comparative Proteomics Kit I: Protein Profiler Module Protein Profiler Kit Instructors Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller E...

Next-Generation Sequencing (NGS) Next Gen Sequencing is an umbrella term describing a collection of high-throughput methodologies and platforms used for the determination of the sequence of base pairs in DNA or RNA. Application Notes Literature Pu...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Advantages of Removing Downstream Bottlenecks Eliminating downstream process bottlenecks has various advantages to establishing a promising future for biopharmaceutical manufacturing. By addressing bottlenecks such as inefficient purification step...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...