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PRODUCT CHECKLIST ☐ Cell Biology Antibodies Cell Culture Equipment Cellular Assays and Signal Transduction Extracellular Matrices and Coated Plates Immunoassays Media Classical Mycoplasma Detection and Removal Serum Serum-Free Specialty Supplement...

Proteomics PROTEIN EXPRESSION ☐ Protein Expression Mammalian Routine Tightly Controlled Ultra-Pure ☐ Prokaryotic Cells Competent Cells Electroporation Induction Reagents PET Vectors for Gateway Cloning ☐ Transfection Insect Cells Mammalian Cells ☐...

PowerPoint Presentation pGLO™ Transformation and Purification of Green Fluorescent Protein (GFP) Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller Education Cente...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

Using Type 1 Water (18.2 MΩ-cm) for High Performance Liquid Chromatography in the Food & Beverage Industry Tammy Goodman Commercial Manager Water Purification Thermo Scientific High Performance Liquid Chromatography (HPLC) is widely used in the fo...

LABORATORY CHECKLIST Clinical ☐ Blood Bank Blood Typing Tubes Prelabeled Centrifuges Copper Sulfate Solutions Crossmatch Racks Dilution Tubes Disposable Culture Tubes Disposable Pasteur Pipets Disposable Transfer Pipets Dry Bath/Block Heaters Exam...

LABORATORY CHECKLIST Clinical ☐ Blood Bank Blood Typing Tubes Prelabeled Centrifuges Copper Sulfate Solutions Crossmatch Racks Dilution Tubes Disposable Culture Tubes Disposable Pasteur Pipets Disposable Transfer Pipets Dry Bath/Block Heaters Exam...

33 LC Colum n Selection – C18 Reversed-Phase M aterials www.hichrom.co.uk • sales@hichrom.co.uk • Tel: +44 (0) 118 930 3660 Hichrom Lim ited C18 Reversed-Phase Materials SPECIFICATIONS OF C18 BONDED RP MATERIALS Octadecyl (ODS) or C18 bonded phase...

Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

Click to edit Master title style Got Protein? Testing the protein content of common foods Bradford Protein Assay Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller...

PowerPoint Presentation Comparative Proteomics Kit I: Protein Profiler Module Protein Profiler Kit Instructors Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller E...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

The advent of digital imaging has transformed Western blot detection. Here we outline how Western blotting has changed and review qualities to look for in a digital imager. Chemiluminescence and fluorescence When most people think of imaging their...

Multicolor detection is a powerful application of fluorescent Western blotting. Two or more proteins can be detected on one blot using antibodies labeled with different fluorophores (Figure 1). The ability to simultaneously assay multiple proteins...