You Searched For: Electrophoresis Gels

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

PowerPoint Presentation Forensic DNA Fingerprinting: Using Restriction Enzymes Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller Education Center Science Chair Tr...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...

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PowerPoint Presentation Crime Scene Investigator PCR Basics™ Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller Education Center Science Chair Tracy High School an...

LABORATORY CHECKLIST Genomics ☐ Plasmid Purification Standard High Purity Plasmid Isolation Rapid Plasmid Isolation Endotoxin-Free Plasmid Isolation Yeast Plasmid Isolation Colony Screening Column Regeneration Accessories ☐ Genomic DNA Purificatio...

PowerPoint Presentation Comparative Proteomics Kit I: Protein Profiler Module Protein Profiler Kit Instructors Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller E...

PowerPoint Presentation pGLO™ Transformation and Purification of Green Fluorescent Protein (GFP) Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller Education Cente...

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LABORATORY CHECKLIST Genomics ☐ Plasmid Purification Accessories Colony Screening Column Regeneration Endotoxin-Free Plasmid Isolation Rapid Plasmid Isolation Standard High Purity Plasmid Isolation Yeast Plasmid Isolation ☐ Genomic DNA Purificatio...

PowerPoint Presentation Comparative Proteomics Kit II: Western Blot Module Comparative Proteomics Kit II: Western Blot Module Instructors Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

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Normalization is critical for obtaining accurate quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel and for differences in transfer efficiency across a blot. Traditionally protein ...

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