You Searched For: Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.


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Catalog Number: (CA97067-926)
Supplier: Cytiva
Description: Capto Butyl is a hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification, Their combination of high capacity, high flow rate and low backpressure reduces process cycle times.

Supplier: Cytiva
Description: Sephadex G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.
Supplier: Cytiva
Description: Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.
Supplier: Cytiva
Description: Ni Ni Sepharose High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Catalog Number: (CA95016-858L)
Supplier: Cytiva
Description: Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

Supplier: Cytiva
Description: DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
Supplier: Cytiva
Description: Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).

SDS

Catalog Number: (CA95017-040)
Supplier: Cytiva
Description: Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

Catalog Number: (CA97067-754)
Supplier: Cytiva
Description: CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.

Catalog Number: (CA97067-872)
Supplier: Cytiva
Description: Phenyl Sepharose High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.

Supplier: Cytiva
Description: Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity
Supplier: Cytiva
Description: Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns such as Superdex, Superose, and Sephacryl.
Supplier: Cytiva
Description: Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain.
Supplier: Cytiva
Description: Sephadex G-100 is useful for desalting and buffer exchange of very large biomolecules.
Catalog Number: (CA95016-818L)
Supplier: Cytiva
Description: CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

Supplier: Cytiva
Description: Sephadex G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.
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