Human Recombinant N-Acetylglucosamine-6-Sulfatase (from Cells)

Supplier: Prosci

91-325
75789-326EA 1536.47 CAD
75789-326
Human Recombinant N-Acetylglucosamine-6-Sulfatase (from Cells)
Enzymes

N-Acetylglucosamine-6-Sulfatase is a member of the Sulfatase family


This recombinant protein can be used for biological assays. For research use only.


Fusion-Tag: C-6 His tag


N-Acetylglucosamine-6-Sulfatase is required for the lysosomal degradation of the Glycosaminoglycans (GAG) Heparan Sulfate and Keratan Sulfate. N-Acetylglucosamine-6-Sulfatase hydrolyzes the 6-Sulfate groups of the N-Acetyl-D-Glucosamine 6-Sulfate units of Heparan Sulfate and Keratan Sulfate. N-Acetylglucosamine-6-Sulfatase binds 1 Calcium ion per subunit. N-Acetylglucosamine-6-Sulfatase deficiency are the cause of Mucopolysaccharidosis Type 3D (MPS3D), an inborn error leading to lysosomal accumulation of heparan sulfate. MPS3D has profound mental deterioration, hyperactivity, and relatively mild somatic manifestations.

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SPECIFICATIONS

Enzyme name N-Acetylglucosamine-6-Sulfatase
Environmentally Preferable
Enzyme synonyms N-Acetylglucosamine-6-Sulfatase, Glucosamine-6-Sulfatase, G6S, GNS
Enzyme type Recombinant
Source Cells
Species Human
Endotoxin content <0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Sequence Val37-Leu552
Molecular weight 59.35 kD
Purity >95% as determined by reducing SDS-PAGE
Storage conditions Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
Formulation Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested applications Bioassay
Tag sequence C-6 His tag

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