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Sodium dihydrogen phosphate monohydrate, granulated, BiotechGrade

Description: Sodium Phosphate Monobasic, Monohydrate, Granular, BiotechGrade is a reagent with very high buffering capacity widely used in molecular biology, biochemistry and chromatography. Spectrum offers highly pure reagents suitable for biochemical research and analysis that are absent of inhibitors.
Catalog Number: 75812-270
Supplier: Spectrum Chemicals
Image Unavailable-CA1.19754.0250

Dipotassium hydrogen orthophosphate trihydrate, LiChropur™ buffer substance, for chromatography, Supelco®

Description: Di-Potassium hydrogen phosphate trihydrate buffer substanceApplication:for chromatography LiChropur
Catalog Number: CA1.19754.0250
Supplier: MilliporeSigma

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Product Image-CAJT4043-1

Glycerine, anhydrous ≥99.5%, Ultrapure Bioreagent for biotechnology, for electrophoresis, J.T.Baker®

Description: Glycerol, anhydrous, Ultrapure Bioreagent, for enzyme stabilisation, freezing and electrophoresis buffers.
Catalog Number: CAJT4043-1
Supplier: AVANTOR PERFORMANCE MATERIAL LLC

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Image Unavailable-77392-590

Sulfo-SMA Membrane protein solubilization & stabilization

Description: Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
Catalog Number: 77392-590
Supplier: CUBE BIOTECH
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Mouse Transferrin, Biotin

Description: Produced through a multi-stage process that includes delipidation, salt fractionation, ion-exchange chromatography, gel filtration, and affinity chromatography. No contaminating proteins are observed when assayed at a protein concentration of 20mg/mL against anti-whole serum or anti-fragment specific antisera. All immunoglobulin fragments are prepared from highly purified, whole molecules subject to enzymatic digestion.
Catalog Number: CA012-0634
Supplier: Rockland Immunochemical
Product Image-CA10498-040

Accessories for ÄKTA Start

Catalog Number: CA10498-040
Supplier: Cytiva
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Goat Peroxidase

Description: Suitable for use in peroxidase enzymatic labeling experiments.
Catalog Number: CAP100-0005
Supplier: Rockland Immunochemical
Image Unavailable-CA010-0634

Mouse transferrin, Biotin

Description: Produced through a multi-stage process that includes delipidation, salt fractionation, ion-exchange chromatography, gel filtration, and affinity chromatography. No contaminating proteins are observed when assayed at a protein concentration of 20mg/mL against anti-whole serum or anti-fragment specific antisera. All immunoglobulin fragments are prepared from highly purified, whole molecules subject to enzymatic digestion.
Catalog Number: CA010-0634
Supplier: Rockland Immunochemical
Image Unavailable-CA1.19753.0250

Disodium hydrogen phosphate dihydrate ≥99.0% (by acidimetry), LiChropur™ buffer substance, for chromatography, Supelco®

Description: Tlc Silica Gel 60 F254 25 Aluminium Sheets 10 X 20 Cm
Catalog Number: CA1.19753.0250
Supplier: MilliporeSigma

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Image Unavailable-CA95043-546

Accessories for Chelating Resin for Immobilized Metal Affinity Chromatography Purification, G-Biosciences

Description: (250mM Imidazole 300mM NaCl 50mM Sodium phosphate pH 8.0) 100ml
Catalog Number: CA95043-546
Supplier: G-Biosciences

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Product Image-CA95017-621L

illustra MicroSpin G-25 Columns, Cytiva

Description: For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.
Catalog Number: CA95017-621L
Supplier: Cytiva

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Product Image-CA95016-746L

Sephadex™ G-10 Gel Filtration Media, Cytiva

Description: Sephadex G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.
Catalog Number: CA95016-746L
Supplier: Cytiva

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Product Image-CA95016-894L

DNA Clean Up, Sephadex® G-50 DNA Grade F, Cytiva

Description: Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.
Catalog Number: CA95016-894L
Supplier: Cytiva

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Product Image-76371-736

VWR® PBS-T (Phosphate Buffered Saline - Tween-20) Buffer Powder Packs

Description: VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.
Catalog Number: 76371-736
Supplier: VWR International

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Product Image-76371-738

VWR® TBS (TRIS Buffered Saline) Buffer Powder Packs

Description: VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.
Catalog Number: 76371-738
Supplier: VWR International
Product Image-76371-722

VWR® TAE Buffer Powder Packs

Description: VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.
Catalog Number: 76371-722
Supplier: VWR International

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