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Description: Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
Catalog Number: 77392-588
Supplier: CUBE BIOTECH


Description: Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
Catalog Number: 77392-594
Supplier: CUBE BIOTECH


Description: Easily customize strainer system to suit your application.
Catalog Number: MFLX29595-09
Supplier: VWR International


Description: Sequence: Sulfo-N-succinimidyl 4-maleimidobutyrate sodium salt
Synonym(s): Sulfo-GMBS sodium salt
Catalog Number: Q-2770.0050BA
Supplier: Bachem Americas


Description: Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Catalog Number: CAPI21335
Supplier: Thermo Scientific

Description: Thermo Scientific Pierce Premium Grade Sulfo-NHS-LC-Biotin is our highest quality formulation of this popular amine-reactive biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount.
Catalog Number: CAPIPG82075
Supplier: Thermo Scientific

Description: Ideal for enzyme-antibody crosslinking.
Catalog Number: CA82022-426
Supplier: G-Biosciences


Description: Thermo Scientific Pierce Sulfo-SIAB a water-soluble, mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via sulfo-N-hydroxysuccinimide (sulfo-NHS) ester and iodoacetyl reactive groups.
Catalog Number: CAPI22327
Supplier: Thermo Scientific

Description: N-(ε-Maleimidocaproyloxy) sulfo succinimide ester Heterobifunctional Cross Linker.
Catalog Number: CA82022-802
Supplier: G-Biosciences


Description: Thermo Scientific Pierce Premium Grade Sulfo-SMCC is our highest quality formulation of amine-to-sulfhydryl crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.
Catalog Number: CAPIPG82086
Supplier: Thermo Scientific

Description: CAS Number: 121461-69-6
Molecular Formula: C14H17BF2N2O6S2
Molecular Weight: 466.19
Purity/Analysis Method: >96.0% (HPLC)
Form: Crystal
Lambda max.: 491 nm (H2O)
Catalog Number: TCD4593-200MG
Supplier: TCI America

SDS


Description: Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Catalog Number: CAPI21217
Supplier: Thermo Scientific

Description: Thermo Scientific Pierce Sulfo-SDA (Sulfo-NHS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 3.9Å spacer arm separates the two photoreactive groups.
Catalog Number: CAPI26173
Supplier: Thermo Scientific

Description: Ponceau S, Proteomics Grade
Catalog Number: 97063-650
Supplier: VWR

Description: CAS Number: 92921-24-9
MDL Number: MFCD00054978
Molecular Formula: C16H18N2O9S
Molecular Weight: 436.37
Form: Crystal
Color: White
Storage Temperature: <0°C
Catalog Number: TCS0883-20MG
Supplier: TCI America

Description: Thermo Scientific Pierce Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.
Catalog Number: CAPI26174
Supplier: Thermo Scientific

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