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Image Unavailable-TCB3194-5G

Coomassie® brilliant blue R-250

Catalog Number: (TCB3194-5G)
Supplier: TCI America
Description: CAS Number: 6104-59-2
MDL Number: MFCD00041762
Molecular Formula: C45H44N3NaO7S2
Molecular Weight: 825.97
Form: Crystal
Color: Deep Red

SDS Certificates


Image Unavailable-CAPI22585

DSP (Dithiobis(succinimidyl propionate)), Premium Grade, Pierce™

Supplier: Thermo Scientific
Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

SDS Certificates

Image Unavailable-77392-528

AASTY 6-45

Supplier: CUBE BIOTECH
Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-45 - are highly alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-45 gets it's name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Image Unavailable-CA-EM3340

Coomassie® brilliant blue R-250, OmniPur®

Catalog Number: (CA-EM3340)
Supplier: MilliporeSigma
Description: Powder. Use for post-electrophoresis staining of proteins in applications requiring a high degree of sensitivity and/or solubility.

SDS Certificates


Image Unavailable-CAPI20278

Coomassie® brilliant blue R-250 65% (dye basis) protein stain

Catalog Number: (CAPI20278)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Coomassie Brilliant Blue R-250 is one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains.

SDS Certificates


Product Image-CA95043-420

Coomassie® brilliant blue R-250 electrophoresis gel stain

Catalog Number: (CA95043-420)
Supplier: G-Biosciences
Description: G-Biosciences' Coomassie Brilliant Blue (CBB) G-250 and R-250 Stain are based on a colloidal Coomassie stain. CBB G-250 and R-250 stain proteins with high band visibility. The staining of gels with CBB G-250 and R-250 allows the examination of protein bands even during the staining process.

SDS


Product Image-CA95043-426

Coomassie® brilliant blue R-250 solution electrophoresis gel stain, ready-to-use

Catalog Number: (CA95043-426)
Supplier: G-Biosciences
Description: G-Biosciences' Coomassie Brilliant Blue (CBB) G-250 and R-250 Stain are based on a colloidal Coomassie stain. CBB G-250 and R-250 stain proteins with high band visibility. The staining of gels with CBB G-250 and R-250 allows the examination of protein bands even during the staining process.

SDS


Image Unavailable-CAAAAB21553-36

(1R)-(-)-Camphor-10-sulfonic acid 98%

Catalog Number: (CAAAAB21553-36)
Supplier: Thermo Scientific Chemicals
Description: (1R)-(-)-Camphor-10-sulfonic acid 98%

Certificates


Image Unavailable-ABCA_AB223602-5MG

PFI-3, BROMODOMAIN AND SMA AB223602-5MG

Catalog Number: (ABCA_AB223602-5MG)
Supplier: Abcam
Description: PFI-3, BROMODOMAIN AND SMA AB223602-5MG

New Product


Image Unavailable-77663-904

IN-LINE FIBER OPTIC HOLDER SMA

Catalog Number: (77663-904)
Supplier: WORLD PRECISION INSTRUMENTS LLC
Description: IN-LINE FIBER OPTIC HOLDER SMA

New Product


Image Unavailable-MSPP-CCT123110

SULFO DBCO-PEG 4 MALEIMIDE

Catalog Number: (MSPP-CCT123110)
Supplier: Vector Laboratories
Description: SULFO DBCO-PEG 4 MALEIMIDE

New Product


Image Unavailable-MSPP-ESK09

KIT BNC AND SMA ADAPTER ESSENTIAL 76PCS

Catalog Number: (MSPP-ESK09)
Supplier: THORLABS INC MS
Description: KIT BNC AND SMA ADAPTER ESSENTIAL 76PCS

New Product


Image Unavailable-ABCA_AB145607-50MG

SULFO-SMCC, AMINE-TO-SULF AB145607-50MG

Catalog Number: (ABCA_AB145607-50MG)
Supplier: Abcam
Description: SULFO-SMCC, AMINE-TO-SULF AB145607-50MG

New Product


Image Unavailable-103784-902

C12 DI-SULFO GALACTOSYL(B) CERAMIDE 1MG

Catalog Number: (103784-902)
Supplier: Avanti Polar Lipids Inc
Description: C12 DI-SULFO GALACTOSYL(B) CERAMIDE 1MG
Image Unavailable-ABCA_AB145608-500M

SULFO-NHS (SULFO-N-HYDRO AB145608-500MG

Catalog Number: (ABCA_AB145608-500M)
Supplier: Abcam
Description: SULFO-NHS (SULFO-N-HYDRO AB145608-500MG

New Product


Image Unavailable-ABCA_AB145611-100M

SULFO-NHS-LC-BIOTIN, BIO AB145611-100MG

Catalog Number: (ABCA_AB145611-100M)
Supplier: Abcam
Description: SULFO-NHS-LC-BIOTIN, BIO AB145611-100MG

New Product


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • issued document with your organization's Federal Tax ID Number
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