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Catalog Number: (CAPI23013)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.

Catalog Number: (CAPI20589)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce DST, also called disuccinimidyl tartrate, is a water-soluble crosslinker that contains amine-reactive NHS ester ends around a 4-atom spacer arm, whose central diol can be cleaved by periodate oxidation.

Catalog Number: (103010-372)
Supplier: Anaspec Inc
Description: Biotin, SE is amino-reactive biotinylating reagent for peptides and proteins; it has a better avidin-binding affinity than biotin. Biotin, SE (d-Biotin-N-hydroxysuccinimide ester, Biotin, NHS ester) contains a five-carbon spacer arm that reduces steric hindrance associated with binding four biotinylated molecules per one avidin and results in enhanced detection sensitivity.


Catalog Number: (103010-916)
Supplier: Anaspec Inc
Description: An excellent amine-reactive FRET quencher paired with Trp or Tyr.


Catalog Number: (CAPI22363)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).

Catalog Number: (CAPI22589)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.

Catalog Number: (CAPI21578)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

Catalog Number: (CAPI22317)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Sulfo-SMPB is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

SDS


Catalog Number: (CAPI22327)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Sulfo-SIAB a water-soluble, mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via sulfo-N-hydroxysuccinimide (sulfo-NHS) ester and iodoacetyl reactive groups.

Supplier: Thermo Scientific
Description: Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.
Supplier: Thermo Scientific
Description: The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.
Supplier: Anaspec Inc
Description: Although the mixed TAMRA isomers are predominantly used for labeling proteins, the single isomers are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used than 6-TAMRA for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids.

Catalog Number: (CA76102-544)
Supplier: Cytiva
Description: The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

Catalog Number: (CA82022-422)
Supplier: G-Biosciences
Description: (<sup>5</sup>/<sub>6</sub>) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.


Catalog Number: (CAPI21566)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Catalog Number: (CAPI22362)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 angstroms).

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