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SensoLyte® Plus 520 MMP-2 Assay Kit (Fluorimetric and Enhanced Selectivity), AnaSpec Inc.

Description: The SensoLyte® Plus 520 MMP-2 Assay Kit is designed specifically for detecting MMP-2 activity in biological samples, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate, which may contain multiple MMPs. A monoclonal anti-human-MMP-2 antibody is employed to pull down MMP-2 from the mixture first. MMP-2 activity is then quantified by a 5-FAM/QXL® 520 fluorescence resonance energy transfer (FRET) peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-2-induced cleavage of the FRET substrate. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. Ample materials are provided to perform 96 assays in a 96-well format.
Catalog Number: 103010-664
Supplier: Anaspec Inc
Image Unavailable-77661-210

Human Hematopoietic Progenitor Cell Expansion Recombinant Protein Bundle (rh SCF, rh FLT-3 Ligand, rh IL-6, rh IL-3) (from E. coli)

Description: Cytokines for expansion and cell culture of human hematopoietic stem cells to progenitor cells. Hematopoietic stem cells are self renewing pluripotent cells often characterized by the expression of CD34. Studies have shown that decreased expression of CD34 tends to correlate with the decreased self renewal properties and pluripotent potential. Culturing CD34+ cells in a cytokine mixture of SCF, FLT-3 Ligand, IL-6 and IL-3 for short time periods allows rapid expansion of immature and more mature hematopoietic progenitor cells in cord blood or bone marrow preparations. If the goal is to only expand the most immature progenitor and stem cells, it is recommended that a combination of TPO, SCF and FLT-3 Ligand are used (Catalog: PB-500-03). Either cytokine combination can also be supplemented with other cytokines to promote specific lineages.
Catalog Number: 77661-210
Supplier: FUJIFILM IRVINE SCIENTIFIC, INC

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Image Unavailable-10772-872

Human Recombinant VAP-1 (from CHO Cells)

Description: VAP-1 is a type II membrane cell adhesion protein belonging to the copper/topaquinone oxidase family. It is primarily expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. VAP-1 can catalyze the oxidative deamination of low molecular weight amines, and plays an important role in the migration of lymphocytes to inflamed tissue. Inhibition of VAP-1 can protect against inflammation-related damage to certain injured tissues. Additionally, VAP-1 can function as a significant prognostic marker for certain cancers and cardiovascular diseases. Recombinant Human VAP-1 is a mixture of monomeric and disulfide-linked homodimeric forms of a 737 amino acid polypeptide, corresponding to amino acids 27 to 763 of the VAP-1 precursor. The calculated molecular weight of Recombinant Human VAP-1 is 81.8 kDa.
Catalog Number: 10772-872
Supplier: Peprotech
Product Image-MSPP-100-1385

HIV-1 (B Gag) Peptide Pool

Description: Stimulating T cells with HIV-1 (B Gag) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HIV-1 (B Gag) Peptide Pool is a lyophilized mixture of 123 peptides from the gag polyprotein of human immunodeficiency virus 1 (HIV-1). The pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 500 on gag polyprotein. The HIV-1 gag polyprotein is a key mediator of viral particle assembly (Campbell and Rein; Dong <i>et al.</i>), and together with the endosomal sorting complexes required for transport (ESCRT), results in budding during virion release (Carlson <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
Catalog Number: MSPP-100-1385
Supplier: STEMCELL Technologies

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Anti-CD86 (B7-2) (GL-1) Rat Monoclonal Antibody (PE (Phycoerythrin)) [clone: GL-1 (GL1)]

Description: The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells. The memory CD4+ T lymphocytes express CD86 (as mRNA and protein). CD86, and the B7-1 (CD 80) molecule, are ligands for CD152 and CD28, and influence the costimulatory interactions between lymphocytes B and T. B7-2 is also involved in the mouse natural killer cell-mediated cytotoxicity. The GL-1 antibody blocks the mixed lymphocyte reaction (MLR) and inhibits the T-cells stimulation by antigen-presenting cells. Mixtures of anti-B7-1 antibody and GL-1 were reported to inhibit the interaction between CD125 and its ligand with the in vivo priming of cytotoxic T lymphocytes.
Catalog Number: 75843-002
Supplier: BIOGEMS INTERNATIONAL INC.
Image Unavailable-75843-010

Anti-CD86 Rat Monoclonal Antibody (APC (Allophycocyanin)) [clone: GL-1 (GL1)]

Description: The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells. The memory CD4+ T lymphocytes express CD86 (as mRNA and protein). CD86, and the B7-1 (CD 80) molecule, are ligands for CD152 and CD28, and influence the costimulatory interactions between lymphocytes B and T. B7-2 is also involved in the mouse natural killer cell-mediated cytotoxicity. The GL-1 antibody blocks the mixed lymphocyte reaction (MLR) and inhibits the T-cells stimulation by antigen-presenting cells. Mixtures of anti-B7-1 antibody and GL-1 were reported to inhibit the interaction between CD125 and its ligand with the in vivo priming of cytotoxic T lymphocytes.
Catalog Number: 75843-010
Supplier: BIOGEMS INTERNATIONAL INC.
Product Image-MSPP-100-1410

Clostridium (Tetanus Toxin) Peptide Pool

Description: Stimulating T cells with Clostridium (Tetanus Toxin) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. Clostridium Peptide Pool is a lyophilized mixture of 326 peptides from tetanus toxin of Clostridium tetani (strain Massachusetts/E88), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 - 1315 on tetanus toxin. Tetanus toxin entry into central neurons is mediated by the synaptic vesicle-binding protein SV2 (Yeh <i>et al.</i>), where it selectively cleaves synaptobrevin (Link <i>et al.</i>; Schiavo <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
Catalog Number: MSPP-100-1410
Supplier: STEMCELL Technologies

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Product Image-MSPP-100-1390

EBV (GP350/GP340) Peptide Pool

Description: Stimulating T cells with EBV (GP350/340) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (GP350/340) Peptide Pool is a lyophilized mixture of 224 peptides from envelope glycoprotein GP350 (GP350/340) of Epstein-Barr virus (EBV; strain B95-8), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 - 907 on GP350/340. GP350 meditates EBV attachment to B lymphocytes via binding to the cell surface receptor CR2 (also known as C3d receptor or CD21) (Nemerow <i>et al.</i>; Tanner <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
Catalog Number: MSPP-100-1390
Supplier: STEMCELL Technologies

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Mouse Recombinant Mouse Hpc Expansion PB (from E. coli)

Description: Cytokines for expansion and cell culture of mouse hematopoietic stem cells to progenitor cells. Hematopoietic stem cells are self renewing pluripotent cells often characterized by the expression of CD34. Studies have shown that decreased expression of CD34 tends to correlate with the decreased self renewal properties and pluripotent potential. Culturing CD34+ cells in a cytokine mixture of SCF, FLT-3 Ligand, IL-6 and IL-3 for short time periods allows rapid expansion of immature and more mature hematopoietic progenitor cells in cord blood or bone marrow preparations. If the goal is to only expand the most immature progenitor and stem cells, it is recommended that a combination of TPO, SCF and FLT-3 Ligand are used (Catalog: PB-500-04). Either cytokine combination can also be supplemented with other cytokines to promote specific lineages.
Catalog Number: 77661-212
Supplier: FUJIFILM IRVINE SCIENTIFIC, INC

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Product Image-MSPP-100-1406

HHV1 (gD) Peptide Pool

Description: Stimulating T cells with HHV1 (gD) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV1 (gD) Peptide Pool is a lyophilized mixture of 96 peptides from envelope glycoprotein D of human herpesvirus 1 (HHV1; strain Patton), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 - 394 on gD. gD is required at the first stage of virus-induced membrane fusion (Subramanian and Geraghty), which likely occurs via binding to receptors such as nectin-1 (Giovine <i>et al.</i>) and HVEM (Connolly <i>et al.</i>) on cell surfaces. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
Catalog Number: MSPP-100-1406
Supplier: STEMCELL Technologies

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Image Unavailable-77661-206

Human Recombinant Human Hsc Expansion PB (from E. coli)

Description: Cytokines for expansion and cell culture of human hematopoietic stem cells. Hematopoietic stem cells are self renewing pluripotent cells often characterized by the expression of CD34. Studies have shown that decreased expression of CD34 tends to correlate with decreased self renewal properties and pluripotent potential. Culturing CD34+ cells in a cytokine mixture of TPO, SCF, and FLT-3 Ligand for short time periods allows CD34+ cells to proliferate without decreasing CD34 expression, thus retaining their stem cell properties. The combination of these cytokines allows for the <i>ex vivo</i> expansion of the most immature hematopoietic stem cells in cord blood or bone marrow preparations, but can be supplemented with other cytokines to promote specific lineages. For example, GM-CSF and/or G-CSF can be added to promote monocytes and granulocytes while EPO can be added to promote erthroid cells.
Catalog Number: 77661-206
Supplier: FUJIFILM IRVINE SCIENTIFIC, INC

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Product Image-10665-256

Anti-IFNalpha Rabbit Polyclonal Antibody

Description: The genes encoding type I interferons (IFNs), which include 14 IFN-alphagenes (two of which are IFN-alpha1 and IFN-alpha13), one IFN-∫ gene, one IFN-w (also known as IFN-alpha II1) gene and a number of IFN-w pseudogenes, are clustered on human chromosome 9. IFN-alpha and -beta are cytokines that are widely known to induce potent antiviral activity. They exert a variety of other biological effects, including antitumor and immunomodulatory activities and are increasingly used clinically to treat a range of malignancies, myelodysplasias and autoimmune diseases. IFN-w is antigenically different from human IFN-alpha, IFN-beta or IFN-gamma, but is a component of natural mixtures of IFN species produced by virus-induced leukocytes or Burkitt's lymphoma cells. The type I interferon receptor (IFN-alphaR) interacts with IFN-alpha, IFN-beta and IFN-w, and seems to be a multisubunit receptor.
Catalog Number: 10665-256
Supplier: Bioss
Product Image-MSPP-100-1387

EBV (EBNA-3B) Peptide Pool

Description: Stimulating T cells with EBV (EBNA-3B) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (EBNA-3B) Peptide Pool is a lyophilized mixture of 234 peptides from Epstein-Barr nuclear antigen 3B (EBNA-3B) of Epstein-Barr virus (EBV; strain AG876) and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 946 on EBNA-3B. EBNA-3B is considered non-essential for B cell growth transformation (Chen <i>et al.</i>, 2005; Tomkinson and Kieff), but has been shown to regulate the expression of cellular genes in EBV-infected cells (Chen at al., 2006). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
Catalog Number: MSPP-100-1387
Supplier: STEMCELL Technologies

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Product Image-MSPP-100-1422

SARS-CoV-2 (Spike Protein) Omicron XBB.1.5.X Peptide Pool

Description: Stimulating T cells with SARS-CoV-2 (Spike Protein) Omicron XBB.1.5.X Mutation peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. This pool is provided as two lyophilized mixtures (subpools) from the spike glycoprotein of SARS-CoV-2 Omicron XBB.1.5.X subvariant. The subpools contain 158 and 157 peptides, respectively, for a total of 315 peptides, and consist of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 1269 on the spike protein. Through interactions with the angiotensin-converting enzyme 2 (ACE2) receptor, the spike protein enables the virus to attach to the cell membrane, and plays a critical role in viral entry (Hoffmann <i>et al.</i>; Walls <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
Catalog Number: MSPP-100-1422
Supplier: STEMCELL Technologies

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Product Image-MSPP-100-1388

EBV (LMP1) Peptide Pool

Description: Stimulating T cells with EBV (LMP1) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (LMP1) Peptide Pool is a lyophilized mixture of 94 peptides from latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV; strain B95-8), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 - 386 on LMP1. LMP1 is essential for EBV-mediated growth transformation of B cells (Kaye <i>et al.</i>) and has been shown to downregulate toll-like receptor 9 (TLR9) expression in infected cells, a mechanism the virus may use to escape host innate immune recognition (Fathallah <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
Catalog Number: MSPP-100-1388
Supplier: STEMCELL Technologies

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Product Image-MSPP-100-1401

HHV6 (U54) Peptide Pool

Description: Stimulating T cells with HHV6 (U54) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV6 (U54) peptide pool is a lyophilized mixture of 112 peptides from protein U54 of human herpesvirus 6B (HHV-6B; strain Z29), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 459 on U54. U54 is a tegument transactivator and is suggested to be a homolog of the CMV UL82/83 (pp65/72K) protein (Isegawa <i>et al.</i>; Nicholas). In infected T cells, U54 has been shown to inhibit gene transcription of interleukin 2 (IL-2), the T cell growth factor, which likely contributes to viral persistence (Iampietro <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
Catalog Number: MSPP-100-1401
Supplier: STEMCELL Technologies

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