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Catalog Number: (76733-484)
Supplier: ANTIBODIES.COM LLC
Description: Mouse POMGNT1 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the <i>in vitro</i> quantitative determination of mouse POMGNT1 in serum, plasma, tissue homogenates, and other biological fluids.


Catalog Number: (76742-354)
Supplier: ANTIBODIES.COM LLC
Description: Human POMGNT1 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the <i>in vitro</i> quantitative determination of human POMGNT1 in serum, plasma, tissue homogenates, and other biological fluids.


Catalog Number: (76743-582)
Supplier: ANTIBODIES.COM LLC
Description: Human MASP2 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the <i>in vitro</i> quantitative determination of human MASP2 in serum, plasma, tissue homogenates, and other biological fluids.


Catalog Number: (77211-000)
Supplier: ANTIBODIES.COM LLC
Description: Human MASP2 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the <i><i>in vitro</i></i> quantitative determination of human MASP2 in serum, plasma, and other biological fluids.


Catalog Number: (10073-156)
Supplier: Prosci
Description: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy (4). This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells (5). ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7 (6).


Catalog Number: (CAGKE-5006A)
Supplier: AGILENT TECHNOLOGIES, INC (CSD) CA
Description: AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).


Catalog Number: (10771-888)
Supplier: Peprotech
Description: Recombinant Human IGF-I LR3, 9.1 kDa, single, non-glycosylated polypeptide chain containing 83 amino acid residues. Animal Free, Source: E.coli, Purity: > 98% by SDS-PAGE gel and HPLC analyses. Synonym: Long R3 IGF-I, Insulin-like Growth Factor-I, Pack Size: 1MG


Catalog Number: (CAGKE-5006D)
Supplier: AGILENT TECHNOLOGIES, INC (CSD) CA
Description: AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).


Catalog Number: (76203-064)
Supplier: MP Biomedicals
Description: Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. It is a lyophilized powder containing sucrose, sodium chloride and Tris. The predominant form of thrombin in vivo is the zymogen, prothrombin (factor II), which is produced in the liver. The concentration of prothrombin in normal human plasma is 5–10 mg/dL. Prothrombin is a glycoprotein with a glycan content of ~12%.


Catalog Number: (102830-006)
Supplier: Bulldog Bio
Description: PNGase F PRIME is a mutant recombinant PNGase F cloned from Flavobacterium meningosepticum and expressed and purified from E. coli. The proprietary changes made to PNGase F have been shown to have unique characteristics when compared to other commercially-available sources of PNGase F. Data generated by independent labs shows that PRIME works on native glycoproteins and serum glycoproteins in minutes at room temperature. Glycan analysis of the digestion products shows that PNGase F PRIME digestion led to more complete glycan release and also allowed for the cleavage of glycans not released by the commercially-available enzymes when used at the same concentrations with the same digestion conditions. This advancement benefits applications that seek to understand glycobiology in a natural milieu. Preliminary data indicates that PNGase F PRIME has a higher specificity towards complex (tri and tetra-antennary) sialylated structures compared to the commercially sourced enzyme. Additionally, the work presented in this Analytical Chemistry paper utilized PNGase F PRIME for all in situ tissue work as the commercially-available PNGase enzymes did not work on native tissue to allow glycan recognition.


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