You Searched For: Fluorescein-12-dUTP


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Catalog Number: (89165-828)
Supplier: Enzo Life Sciences
Description: Bio-16-dUTP (Biotin-16-2’-deoxyuridine-5’-triphosphate) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.


Catalog Number: (10109-526)
Supplier: Prosci
Description: DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.


Catalog Number: (89165-750)
Supplier: Enzo Life Sciences
Description: Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.


Catalog Number: (89165-752)
Supplier: Enzo Life Sciences
Description: Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.


Catalog Number: (89165-856)
Supplier: Enzo Life Sciences
Description: Aqua 431 [DEAC] dUTP is a coumarin derivative that can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.


Catalog Number: (89165-848)
Supplier: Enzo Life Sciences
Description: Orange 552 dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Orange 552 dUTP can also be used for multicolor fluorescence labeling.


Catalog Number: (89165-814)
Supplier: Enzo Life Sciences
Description: Terminal labeling is an ideal procedure for the labeling of oligonucleotides with haptens such as biotin or fluorescein.


Catalog Number: (89165-846)
Supplier: Enzo Life Sciences
Description: Fluorescein-12-UTP is ideal for in vitro transcription reactions catalyzed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridization to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridization procedures.


Catalog Number: (89165-850)
Supplier: Enzo Life Sciences
Description: Gold 525 [5(6)-Carboxyrhodamine 6G] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.


Catalog Number: (89165-758)
Supplier: Enzo Life Sciences
Description: Gold 550 [Cyanine-3E] dUTP is a cyanine-3 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.


Catalog Number: (89165-786)
Supplier: Enzo Life Sciences
Description: For preparation of Cyanine 3- and Cyanine 5-labeled DNA for hybridization to BAC arrays.The CGH Labeling Kit for BAC Arrays has been optimized to label DNA for dual sample comparative genomic hybridization (CGH) assays on BAC arrays. All required reagents are provided including fully optimized nucleotide mixes that contain either cyanine 3-dUTP or cyanine 5-dUTP.


Catalog Number: (102883-966)
Supplier: R&D Systems
Description: Human IL-12 R beta 1 Fluorescein (Clone 69310), Host: Mouse IgG1, Monoclonal, Immunogen: Mouse myeloma cell line NS0-derived recombinant, Flow Cytometry, 25 Tests


Catalog Number: (76959-108)
Supplier: ANTIBODIES.COM LLC
Description: Anti-p53 Mouse Monoclonal Antibody [clone: BP53-12] (FITC (Fluorescein Isothiocyanate))


Catalog Number: (89165-760)
Supplier: Enzo Life Sciences
Description: Red 650 [Cyanine-5E] dUTP is a cyanine-5 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.


Catalog Number: (89165-858)
Supplier: Enzo Life Sciences
Description: Allylamine-2’-deoxyuridine-5’-triphosphate can replace TTP in reactions where it serves as a substrate for E. coli DNA polymerase 1 (holoenzyme and Klenow fragment), terminal deoxynucloetide transferase, T4 and Taq DNA polymerases, and reverse transcriptases (from ALV and MLV).


Catalog Number: (77131-572)
Supplier: Prosci
Description: Anti-Cd2 Rat Monoclonal Antibody [clone: [12-15]] (FITC (Fluorescein Isothiocyanate))


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