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Product Image-77392-488

PureCube DIBMA Glycerol, Screening Kit, HEPES

Catalog Number: (77392-488)
Supplier: CUBE BIOTECH
Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Image Unavailable-CA8.14734.0100

Diisobutylene mixed isomers zS, Sigma-Aldrich®

Supplier: MilliporeSigma
Description: Diisobutylene mixed isomers zS, Sigma-Aldrich®

SDS Certificates

Image Unavailable-77392-486

PureCube DIBMA Glucosamine, Screening Kit, HEPES

Catalog Number: (77392-486)
Supplier: CUBE BIOTECH
Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Image Unavailable-77392-480

DIBMA 12 in HEPES, PureCube for protein solubilisation

Supplier: CUBE BIOTECH
Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.

Product Image-77392-492

DIBMA glucosamine in HEPES, PureCube for protein solubilisation

Supplier: CUBE BIOTECH
Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.

Image Unavailable-77392-478

DIBMA 10 in HEPES, PureCube for protein solubilisation

Supplier: CUBE BIOTECH
Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.

Product Image-77392-468

PureCube DIBMA 10, Screening Kit, HEPES

Catalog Number: (77392-468)
Supplier: CUBE BIOTECH
Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Product Image-77392-472

PureCube DIBMA 12, Screening Kit, HEPES

Catalog Number: (77392-472)
Supplier: CUBE BIOTECH
Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Product Image-77392-500

DIBMA Screening Kit, HEPES

Catalog Number: (77392-500)
Supplier: CUBE BIOTECH
Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Image Unavailable-77392-496

DIBMA glycerol in HEPES, PureCube for protein solubilisation

Supplier: CUBE BIOTECH
Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.

Image Unavailable-CAAA43760-A7

Copper(II) pyrophosphate hydrate (43,5 - 48,5% P₂O₇)

Supplier: Thermo Scientific Chemicals
Description: Copper(II) pyrophosphate hydrate is used in printed circuit boards. It catalyzes the dimerization of isobutylene to diisobutylene. It is involved in the electrodeposition of copper-zinc alloys based electrolytes. Further, it is used in animal feed as a supplement. In addition to this, it is used in plating of copper on plastics, aluminum, and zinc.

Certificates

Product Image-77392-596

Sulfo-DIBMA Membrane protein solubilisation & stabilisation

Supplier: CUBE BIOTECH
Description: Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)

Product Image-77392-860

SARS-CoV-2 Recombinant SARS CoV-2 Spike Protein in DIBMA Glycerol (from
Hek293 Expi cells)

Supplier: CUBE BIOTECH
Description: The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

Product Image-77392-566

Synthetic Nanodisc Screening Kit MINI

Catalog Number: (77392-566)
Supplier: CUBE BIOTECH
Description: Synthetic copolymers are capable of solubilizing and stabilizing membrane proteins without the need for detergents. They have the unique capacity to extract the membrane protein of interest while maintaining a near-native environment.
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