Print…

You Searched For: Masterflex+Luer+Fittings


64,400  results were found

Refine Results Sort by

Sort Results

List View Easy View
SearchResultCount:"64400"

Rate These Search Results

Image Unavailable-77392-478

DIBMA 10 in HEPES, PureCube for protein solubilisation

Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.
Catalog Number: 77392-478
Supplier: CUBE BIOTECH
Product Image-77392-468

PureCube DIBMA 10, Screening Kit, HEPES

Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Catalog Number: 77392-468
Supplier: CUBE BIOTECH
Image Unavailable-77392-594

Sulfo-DIBMA Membrane protein solubilization & stabilization

Description: Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
Catalog Number: 77392-594
Supplier: CUBE BIOTECH
Product Image-77392-488

PureCube DIBMA Glycerol, Screening Kit, HEPES

Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Catalog Number: 77392-488
Supplier: CUBE BIOTECH
Image Unavailable-77392-474

DIBMA 12 in HEPES, PureCube for protein solubilisation

Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.
Catalog Number: 77392-474
Supplier: CUBE BIOTECH
Image Unavailable-77392-582

Glyco-DIBMA in HEPES, PureCube for protein solubilisation

Description: Glyco-DIBMA is a modified version of DIBMA with a sugar side-chain added to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose. DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.
Catalog Number: 77392-582
Supplier: CUBE BIOTECH
Image Unavailable-77392-484

DIBMA glucosamine in HEPES, PureCube for protein solubilisation

Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.
Catalog Number: 77392-484
Supplier: CUBE BIOTECH
Image Unavailable-77392-486

PureCube DIBMA Glucosamine, Screening Kit, HEPES

Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Catalog Number: 77392-486
Supplier: CUBE BIOTECH
Image Unavailable-77392-490

DIBMA glycerol in HEPES, PureCube for protein solubilisation

Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Catalog Number: 77392-490
Supplier: CUBE BIOTECH
Product Image-77392-472

PureCube DIBMA 12, Screening Kit, HEPES

Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Catalog Number: 77392-472
Supplier: CUBE BIOTECH
Image Unavailable-77132-292

SARS-CoV-2 (COVID-19, 2019-nCoV) Recombinant full length spike protein in DIBMA Glycerol (from HEK293 cells)

Description: SARS-CoV-2 (COVID-19, 2019-nCoV) Recombinant full length spike protein in DIBMA Glycerol (from HEK293 cells)
Catalog Number: 77132-292
Supplier: Prosci
Product Image-77392-500

DIBMA Screening Kit, HEPES

Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Catalog Number: 77392-500
Supplier: CUBE BIOTECH
Product Image-77392-592

Sulfo-SMA Membrane protein solubilization & stabilization

Description: Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
Catalog Number: 77392-592
Supplier: CUBE BIOTECH
Product Image-77392-860

SARS-CoV-2 Recombinant SARS CoV-2 Spike Protein in DIBMA Glycerol (from
Hek293 Expi cells)

Description: The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
Catalog Number: 77392-860
Supplier: CUBE BIOTECH
Image Unavailable-77392-856

SARS-CoV-2 Recombinant SARS CoV-2 Spike Protein Set in LMNG Detergent, DIBMA Glycerol, Nanodisc Complex (from HEK293 cells)

Description: The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
Catalog Number: 77392-856
Supplier: CUBE BIOTECH
Product Image-77392-562

SMALP Screening Set

Description: Membrane proteins and their composition are as versatile as the whole cell membrane itself. For this reason, it is impossible to predict with 100% accuracy which SMALP product is best suited for the solubilization and subsequent preparation of your membrane protein of interest. We offer this SMALP Screening Kit that consists of our four individual SMAs. This way, you can conveniently screen for the best SMA for your protein before scaling up your assay. In case you would like to extend your polymer screening beyond the reaches of SMA, we offer our Synthetic Nanodisc Screening Kit, which covers both SMA and DIBMA.
Catalog Number: 77392-562
Supplier: CUBE BIOTECH
945 - 960 of 64,400