You Searched For: Antibody+Purification+Buffers


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Catalog Number: (CA103257-624)
Supplier: New England Biolabs (NEB)
Description: The Protein Deglycosylation Mix II contains all of the enzymes, reagents, and controls needed to remove all N-linked and simple O-linked glycans as well as some complex O-linked glycans.


Catalog Number: (CA76046-292)
Supplier: MP Biomedicals
Description: BioPulverizer™ System II contains 10x2 mL tubes of each of our specialized Lysing Matrices.


Catalog Number: (CA76307-362)
Supplier: New England Biolabs (NEB)
Description: The Monarch® RNA cleanup columns (10 µg) are a component of the Monarch RNA cleanup kit (10 µg) and can be used to purify up to 10 µg of RNA from enzymatic reactions. They are supplied with Monarch® collection tubes II. RNA cleanup binding buffer and Monarch® RNA wash buffer, are also available separately.


Catalog Number: (CAPI44899)
Supplier: Thermo Scientific
Description: The Thermo Scientific CarboxyLink Immobilization Kit is for covalent immobilization of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

SDS


Catalog Number: (CA80500-372)
Supplier: MilliporeSigma
Description: The SpinPrep™ PCR Clean-Up Kit is designed for the rapid purification of DNA amplified in PCR. This procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low-salt buffer. This kit removes DNA polymerases, dNTPs, salts, and > 99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labeling. PCR products from 100 bp to > 12,000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60-90% under standard conditions.

Supplier: Sartorius
Description: LentiSELECT kits offer LentiSELECT 40, LentiSELECT 500 and LentiSELECT 1000 for the purification and concentration of VSV-G pseudotyped lentivirus from 40 to 1000 ml cell culture, leading to purified infective particles. These lentivirus purification and concentration kits offer purified recombinant lentivirus particles for <i>in-vitro</i> transfection a fast, safe and easy to use solution. These kits replace time-consuming ultracentrifugation protocols, which typically take about one day for large sample volumes, thus reducing the purification time to only a few hours.

Catalog Number: (CAPI77720)
Supplier: Thermo Scientific
Description: Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0.5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.

Catalog Number: (CA95017-621L)
Supplier: Cytiva
Description: For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.

Supplier: New England Biolabs (NEB)
Description: The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from previously isolated total RNA.

Catalog Number: (CA95026-726L)
Supplier: Cytiva
Description: The illustra™ GFX™ 96 Purification Kit utilises glass fibre matrix technology in a 96-well format for highly efficient purification of PCR products. Fragments from PCR are captured by the matrix in the presence of a chaotropic salt, and contaminants are conveniently removed by washing the matrix with a buffered ethanol solution.

Supplier: Cytiva
Description: illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.
Supplier: IBI Scientific
Description: I-Blue Midi Plasmid Kits optimize isolation of plasmid DNA from E.coli with a simple lysis, bind, wash, and elute protocol. The kits include I-Blue lysis buffer, which helps prevent common handling errors, ensuring efficient cell lysis and SDS precipitation.

Catalog Number: (10097-428)
Supplier: Proteintech
Description: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYPF tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


Supplier: IBI Scientific
Description: I-Blue Midi Plasmid Endotoxin-Free Kits optimize isolation of plasmid DNA from E.coli with a simple lysis, bind, wash, and elute protocol. The kits include I-Blue lysis buffer, which helps prevent common handling errors, ensuring efficient cell lysis and SDS precipitation.

Supplier: IBI Scientific
Description: The I-Blue Mini Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7ml of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization

Catalog Number: (CA76409-106)
Supplier: Sartorius
Description: Pump tubing set, for Vivapure Adenopack 100, Complete kit for fast purification of up to 1 x 10 raised to 13 adenovirus type 5 particles from 500 ml cell culture, Contains 2 AdenoPACK 100 syringe filter units with Sartobind ion exchange membrane plus all buffers


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