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Image Unavailable-10018-198

Fluorescein-12-dUTP, lyophilized powder

Description: Fluorescein-12-dUTP can be used to synthesize fluorecently labeled DNA probes for in-situ hybridization, microarray or blotting techniques.
Catalog Number: 10018-198
Supplier: Biotium
Image Unavailable-89165-842

Green 496 dUTP, Enzo Life Sciences

Description: Green 496 [5-Fluorescein] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
Catalog Number: 89165-842
Supplier: Enzo Life Sciences
Image Unavailable-89410-674

dUTP CF® Dye Conjugates, Biotium

Description: CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications.
Catalog Number: 89410-674
Supplier: Biotium
Product Image-89155-906

APO-BRDU™ kit, TUNEL Assay

Description: Kit for measuring apoptosis with high sensitivity by Dual Color Flow Cytometry in mammalian cells. Br-dUTP is more readily incorporated into the genome of apoptotic cells than are larger ligands like fluorescein, biotin, etc. This greater incorporation coupled with the use of a fluorescein labelled anti-BrdU monoclonal antibody (Prod. No. ALX-804-197L) gives rise to a stronger signal in flow cytometry.
Catalog Number: 89155-906
Supplier: Enzo Life Sciences
Product Image-10820-968

APO-BRDU™ kit, TUNEL Assay

Description: The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
Catalog Number: 10820-968
Supplier: Tonbo Biosciences
Image Unavailable-10450-100

Anti-DUT Rabbit Polyclonal Antibody (FITC (Fluorescein Isothiocyanate))

Description: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Catalog Number: 10450-100
Supplier: Bioss
Product Image-89410-738

CF® Dye TUNEL Assay Apoptosis Detection Kits, Biotium

Description: These kits use enzymatic labeling of DNA double-strand breaks for fluorescence staining of apoptotic cells in fixed samples or tissue sections by TUNEL.
Catalog Number: 89410-738
Supplier: Biotium
Product Image-89139-668

Reactive Biotin Compounds, Biotium

Description: Biotinylation reagents with a variety of reactive functional groups.
Catalog Number: 89139-668
Supplier: Biotium
Product Image-10820-970

Anti-BrdU Mouse Monoclonal Antibody (FITC (Fluorescein Isothiocyanate)) [clone: PRB-1]

Description: The fragmentation of genomic DNA by cellular nucleases during the later stages of apoptosis is also one of the most easily measured features of apoptotic cells. Nuclease activity generates DNA fragments ranging from ~300 bp to 50 bp in length, resulting in a typical DNA ‘laddering’ appearance when analyzed by agarose gel electrophoresis. These fragments have exposed 3’-hydroxyl (OH) ends which can be labeled with bromolated deoxyuridine triphosphates (Br-dUTP). An enzyme, terminal deoxynucleotidyl transferase (TdT), is used to catalyze the template-independent addition of Br-dUTP to the 3’-OH ends of double or single stranded DNA. This method is often called TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) or end labeling. Sites where the Br-dUTP is incorporated can then be detected with an antibody specific to BrdU. With the APO-BrdUTM Kit cells are first labeled with Br-dUTP, and then sites of incorporation are detected through staining with a FITC anti-BrdU antibody. Samples can then be analyzed via flow cytometry. Samples that are apoptotic will stain brightly with the anti-BrdU antibody due to the substantial number of exposed 3’-OH sites, while cells that are non-apoptotic will not have incorporated significant amounts of Br-dUTP and will stain dimly. The APO-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.
Catalog Number: 10820-970
Supplier: Tonbo Biosciences
Image Unavailable-76414-946

Cyanine 647-dUTP, lyophilized blue solid

Description: Cyanine 647-dUTP can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques.
Catalog Number: 76414-946
Supplier: Biotium
Image Unavailable-10018-196

DEAC-dUTP (diethylaminocoumarin-dUTP), lyophilized powder

Description: DEAC-dUTP (diethylaminocoumarin-dUTP) can be used to synthesize fluorescently labeled DNA probes for in-situ hybridization, microarray or blotting techniques.
Catalog Number: 10018-196
Supplier: Biotium
Product Image-89139-072

Aminoallyl-dUTP (AA-dUTP) sodium salt, lyophilized powder

Description: 5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method.
Catalog Number: 89139-072
Supplier: Biotium
Image Unavailable-76414-944

Cyanine 555-dUTP, lyophilized red solid

Description: Cyanine 555-dUTP can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques.
Catalog Number: 76414-944
Supplier: Biotium
Product Image-77461-524

iFluor® 488 PEG12-dUTP *1 mM in TE Buffer (pH 7.5)*

Description: This iFluor® 488-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™ Alexa Fluor 488-dUTP.
Catalog Number: 77461-524
Supplier: AAT BIOQUEST INC
Product Image-89139-094

Biotin-20-dUTP, Biotium

Description: Biotin-20-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.
Catalog Number: 89139-094
Supplier: Biotium
Product Image-89139-086

5-Bromo-2′-deoxyuridine-5′-triphosphate (5-Bromo-dUTP) 10 mM in TE buffer

Description: 5-Bromo-dUTP is widely used in TUNEL assay to detect apoptosis cells, and it is also a good substrate for reverse transcriptase.
Catalog Number: 89139-086
Supplier: Biotium
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