Human Recombinant CXCL12 (SDF-1a) (from E. coli), Biotinylated

Supplier: CHEMOTACTICS, INC MS

B-CXCL12-100UG B-CXCL12-50UG
MSPP-BCXCL121EA 862.68 CAD
MSPP-BCXCL121 MSPP-BCXCL1250
Human Recombinant CXCL12 (SDF-1a) (from E. coli), Biotinylated
Proteins and Peptides

Functional biotin labeled human CXCL12a chemokine with very low endotoxin levels.


  • Extremely low endotoxin levels (<0.01 EU per 1 μg)
  • Functional activity tested and validated
  • For use in various streptavidin applications: Flow cytometry, microscopy, and other imaging techniques


As a chemoattractant active on T-lymphocytes, monocytes, but not neutrophils,  SDF-1α activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1α (3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1α(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and Tlymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF-1α/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.


Ordering information: Larger sizes available, please request a custom quote.


Delivery information: Shipped lyophilized at room temperature.


Caution: For research use only, not for use in humans.

Order Now

SPECIFICATIONS

Protein/peptide name CXCL12 (SDF-1a)
Environmentally Preferable
Protein synonyms IRH|PBSF|SDF1|TLSF|TPAR1|SCYB12
Protein/peptide type Recombinant
Protein Function Chemokine
Species Human
Source E. coli
Conjugation Biotin
Gene ID Accession # P48061-2 (22-89)
Protease-free Y
Carrier-free Y
Endotoxin-free Y
Endotoxin level Very low
Endotoxin content <0.01 EU per 1 μg of the protein by the LAL method
Biological activity EC50 = 0.5 - 0.9 nM determined by Calcium Flux with human CXCR4 in U937 cells
Purity >97%
Formulation Lyophilized pellet
Molecular weight 10.38 kDa
Reconstitution instructions Spin sample prior to reconstitution. Recommended concentration of 100 μg/ml in sterile water.
Storage conditions Avoid repeated freeze-thaw cycles. 12 months from date of receipt, –20... –70 °C as supplied. ChemoTactics suggests using immediately after reconstitution. 1 month, –20...–70 °C under sterile conditions after reconstitution.
Shipping temperature RT
Tested applications Migration assay

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR