Rat Recombinant TNF-A (from E. coli)

Supplier: VWR International

77752-928 77752-930 77752-932 77752-934 77752-936
77752-928EA 949.5 CAD
77752-928 77752-930 77752-932 77752-934 77752-936
Rat Recombinant TNF-A (from E. coli)
Proteins and Peptides

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2.


  • High quality
  • Low endotoxin
  • Affordable


TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.


Caution: For research use only.

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SPECIFICATIONS

Protein/peptide name TNF-A
Environmentally Preferable
Protein synonyms TNFSF2|Cachectin|DIF|Necrosin|Cytotoxin|Cachexin|TNF
Protein/peptide type Recombinant
Protein Function Cytokine
Species Rat
Source E. coli
Conjugation Unconjugated
Gene ID P16599
Sequence MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
Protease-free N
Animal-free N
Nuclease-free N
Carrier-free Y
Endotoxin-free N
Endotoxin level Low
Endotoxin content Endotoxin LAL (EU/µg) ≤1
Biological activity Bioactivity assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D; ED50 ≤0.05 ng/ml; Bioactivity ≥2.0×10⁷ units/mg
Purity ≥95%
Grade RUO
Formulation 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Molecular weight 17.3 kDa
Reconstitution instructions Sterile water at 0.1 mg/ml
Storage conditions –20 °C
Shipping temperature RT
Tested applications Bioassay

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