Mouse Recombinant IL-10 (from E. coli)

Supplier: VWR International

77752-724 77752-726 77752-728 77752-730
77752-724EA 1743.42 CAD
77752-724 77752-726 77752-728 77752-730
Mouse Recombinant IL-10 (from E. coli)
Proteins and Peptides

Interleukin 10 (IL-10) is an anti-inflammatory cytokine produced by macrophages and type 2 T helper (Th2) cells.  IL-10 inhibits the production of pro-inflammatory cytokines such as interferon gamma (IFN-ƴ), tumor necrosis factor alpha (TNF-α), interleukin 2 (IL-2), interleukin 3 (IL-3), interleukin 4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF), made by macrophages and regulatory T cells.  IL-10 also suppresses antigen presentation on antigen presenting cells, and enhances the survival, proliferation, and antibody production of B cells. Mouse IL-10 is not active on human cells.


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SPECIFICATIONS

Protein/peptide name IL-10 IL-10
Environmentally Preferable
Protein synonyms Cytokine synthesis inhibitory factor|CSIF Cytokine synthesis inhibitory factor|CSIF
Protein/peptide type Recombinant Recombinant
Protein Function Cytokine Cytokine
Species Mouse Mouse
Source E. coli E. coli
Conjugation Unconjugated Unconjugated
Gene ID P18893 P18893
Sequence MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGEKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLQDQGVYKA MNEFDIFINC IEAYMMIKMK S MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGEKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLQDQGVYKA MNEFDIFINC IEAYMMIKMK S
Protease-free N N
Animal-free N N
Nuclease-free N N
Carrier-free Y Y
Endotoxin-free N N
Endotoxin level Low Low
Endotoxin content Endotoxin LAL (EU/µg) ≤1 Endotoxin LAL (EU/µg) ≤1
Biological activity Bioactivity assay: MC/9 cell proliferation; ED50 ≤5 ng/ml; Bioactivity ≥2.0×10⁵ units/mg Bioactivity assay: MC/9 cell proliferation; ED50 ≤5 ng/ml; Bioactivity ≥2.0×10⁵ units/mg
Purity ≥95% ≥95%
Grade RUO RUO
Formulation 10 mM sodium phosphate, pH 7.5 10 mM sodium phosphate, pH 7.5
Molecular weight 18.9 kDa 18.9 kDa
Reconstitution instructions Sterile water at 0.1 mg/ml (reconstitution at concentrations >0.1 mg/ml may result in a precipitate) Sterile water at 0.1 mg/ml (reconstitution at concentrations >0.1 mg/ml may result in a precipitate)
Storage conditions –20 °C –20 °C
Shipping temperature RT RT
Tested applications Bioassay Bioassay

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