Human Recombinant PDGF-BB (from E. coli)

Supplier: VWR International

77750-844 77750-808 77750-810 77750-812
77750-844EA 5298.06 CAD
77750-844 77750-808 77750-810 77750-812
Human Recombinant PDGF-BB (from E. coli)
Proteins and Peptides

Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β, and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.


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  • Low endotoxin

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Caution: For research use only.

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SPECIFICATIONS

Protein/peptide name PDGF-BB
Environmentally Preferable
Protein synonyms GDGF|ODGF
Protein/peptide type Recombinant
Protein Function Cytokine
Species Human
Source E. coli
Conjugation Unconjugated
Gene ID P01127
Sequence MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
Protease-free N
Animal-free N
Nuclease-free N
Carrier-free Y
Endotoxin-free N
Endotoxin level Low
Endotoxin content Endotoxin LAL (EU/µg) ≤0.1
Biological activity Bioactivity assay: NR6R-3T3 cell proliferation; ED50 ≤20 ng/ml; Bioactivity ≥5.0×10⁴ units/mg
Purity ≥95%
Grade RUO
Formulation 10 mM sodium phosphate, pH 7.5
Molecular weight 12.4/24.9 kDa
Reconstitution instructions Sterile water at 0.1 mg/ml
Storage conditions –20 °C
Shipping temperature RT
Tested applications Bioassay

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