Human Recombinant PDGF-AA (from E. coli)
Supplier: VWR International
Platelet-derived growth factor (PDGF) serves as a crucial controller of cell growth, proliferation, and angiogenesis. PDGF exists as a dimeric glycoprotein, comprising two polypeptide chains (A and B) stabilized by disulfide bridges. These chains can combine to form either heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) PDGF-AA is commonly used for differentiation of neural progenitor cells derived from human pluripotent stem cells (hPSC) into oligodendrocyte precursor cells.
- High quality
- Low endotoxin
- Lot-to-lot consistency
- Animal free
- Manufactured following GMP guidelines
The synthesis of PDGF is stimulated by signaling molecules such as IL-1, IL-6, TNF-α, TGF-β, and EGF. PDGF acts as a growth hormone that induces cell division in mesenchymal lineage cells like smooth muscle and glial cells. Furthermore, PDGF is stored within the alpha-granules of platelets and is released when these platelets adhere to injured tissues. The PDGF dimer binds to cell surface receptor tyrosine kinases, specifically PDGFR-α and PDGFR-β. Studies suggest that PDGF-AA plays a pivotal role as an autocrine regulator of vascular endothelial growth factor (VEGF) expression in non-small cell lung carcinomas. Additionally, PDGF-AA facilitates the proliferation of oligodendrocyte progenitor cells and guides their differentiation along the oligodendrocyte lineage through the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2).
Host cell protein: ≤30 ng/ml
Host cell DNA: ≤0.01 ng/ml
Sterility testing: No growth
Mycoplasma: Negative
Caution: For research use or further manufacturing only.
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