The HIS TAG® monoclonal antibody is a mouse monoclonal antibody (IgG₁) directed against the HIS TAG® sequence encoded by many of the NOVAGEN® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5×10⁻⁸ to 1×10⁻⁹ M) enables sensitive, specific detection of HIS TAG® fusion proteins at antibody concentrations of 0.1 to 0.2 g/ml. The HIS TAG® monoclonal antibody binds to N-terminal, C-terminal, and internal HIS TAG® sequences. This antibody also detects the recombinant marker bands in the perfect protein and trail mix western markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, alkali-soluble casein is recommended as a blocking agent. The 100 g package size provides enough purified antibody for 1000 ml of working solution.
Caution: * Not recommended for HRP-based detection of Trail Mix Western Markers.
Type:
Antigen: His-Tag
Clonality: Monoclonal
Clone:
Conjugation:
Epitope:
Host:
Isotype:
Reactivity:
