HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid, powder acid-free, Corning®

Supplier: Corning

61-034-RM 61-034-RO 61-034-RR
CA45000-692EA 250.75 CAD
CA45000-692 CA45000-694 CA45000-696
HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid, powder acid-free, Corning®
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2


Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.

Formula: C₈H₁₈N₂O₄S
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
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