Terrific Broth, IBI Scientific

Supplier: IBI Scientific

IB49140 IB49142 IB49141

89126-196EA 1686.3 CAD

89126-196 89126-192 89126-194

Terrific Broth, IBI Scientific

Media for Microbiology Dehydrated Media for Microbiology

IBI's Terrific Broth is used with glycerol in cultivating recombinant strains of E

IBI's Terrific Broth is composed of 12.0gm Casein Digest Protein, 24.0gm Yeast Extract, 2.3gm Potassium Phosphate (Monobasic), and Potassium Phosphate (Dibasic) with a pH (@25°C) of 7.8 ± 0.2. To prepare Terrific Broth, mix 50.8gm of the medium in 1L purified water until evenly dispersed. Add 4ml of glycerol to the mixture and stir. Heat with repeated stirring to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Store at room temperature, keep tightly sealed, and protect from moisture and light.

coli. Terrific Broth is a highly enriched medium for improving yield in plasmid bearing E. coli. Recombinant strains have an extended growth phase in the medium. The addition of tryptone and yeast extract in the medium will allow higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid.

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