Transformation of Escherichia coli Lab, Edvotek

Supplier: Edvotek

Transformation of <i>Escherichia coli</i> Lab, Edvotek

Transformation of <i>Escherichia coli</i> Lab, Edvotek

221 221

470193-134EA 208.5 CAD

470193-134 470200-568

Transformation of Escherichia coli Lab, Edvotek

Educational Classroom Kits and Activities

Understand the Basics of Genetic Engineering

Guided or open-inquiry
Bactobeads are stable for nine months at room temp
Time required: ~90 minutes over 2-3 days

In this experiment, your students will develop an understanding of bacterial transformation by plasmid DNA by introducing an opportunity to observe an acquired phenotypic trait of the transformed bacterial cells. The presence of blue bacterial colonies visually demonstrates the expression of a specific gene for the Lac+ phenotype.

Kit includes: instructions, BactoBeads™, plasmid DNA, buffer, media, ampicillin, X-Gal, ReadyPour™ agar, petri dishes, sterile pipettes, loops and microtubes.

Required, but not included: micropipette and tips, two water baths (37˚C and 42˚C), incubation oven, thermometer, pipette pumps or bulbs, ice, marking pens, Bunsen burner, hot plate or microwave oven, and hot gloves

Packaging: Some components require refrigerator & freezer storage on arrival.

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