Pierce™ HRP Substrate Solutions, 1-Step™ Slow TMB-ELISA, Thermo Scientific

Supplier: Thermo Scientific
Pierce
34024 34024
CAPI34024EA 315.68 CAD
PI34024 CAPI34024
Pierce™ HRP Substrate Solutions, 1-Step™ Slow TMB-ELISA, Thermo Scientific
Assays ELISAs
The 1-Step™ Slow TMB-ELISA contains soluble TMB (3,3´,5,5´-tetramentylbenzidine) that is ideal for ELISA-based applications.

TMB is a chromogenic substrate for horseradish peroxidase. Once oxidised by the enzyme, this substrate yields a blue product that absorbs at 370 nm and 652 nm. Adding sulfuric or phosphoric acid to stop the reaction changes the product to yellow that absorbs at 450 nm and provides greater sensitivity than the blue product. The 1-Step Slow TMB-ELISA is supplied ready to use with no mixing required.
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