PRP-1 Polymeric Reversed Phase HPLC Columns, Hamilton Company

Supplier: Hamilton
79445 79352 79529 79426 79496 79571 79478 79479 79351 79531 79380 79317 79428 79495 79447 79423 79499 79422 79381 79427 79444 79366 79425 79443
89221-070EA 8335.64 CAD
89221-070 10040-432 89221-062 21480-120 21480-050 21493-100 21480-015 21480-016 21480-017 21480-018 21493-225 89221-148 89221-138 89221-116 21493-464 89221-144 89221-068 89221-112 89221-146 21493-446 89221-140 89221-152 89168-728 89221-098
PRP-1 Polymeric Reversed Phase HPLC Columns, Hamilton Company
Chromatography Columns
Hamilton Company offers one of the most comprehensive selections of chromatography columns in the industry.

  • pH Stable from 1 to 13
  • Polymeric Columns for General Separations
  • Four Particle Sizes: 5, 7, 10, and 12 to 20 µm
  • Custom Hardware Dimensions Available
  • Analytical and Semiprep/Preparative Guard Columns
  • Buffer strength 0.0 - 0.5N

Each column is manufactured under rigorous criteria and tested for resolution and retention time accuracy.

PRP-1 polymeric HPLC columns with 100 Å pore size particles are designed for broad ranging uses, similar to C8 or C18 HPLC materials. Hamilton PRP-1 (Polymeric Reversed Phase) HPLC columns combine the best characteristics of silica-based and polymeric columns to arrive at a column that is highly inert and long-lasting. Quality assurance as well as research and development laboratories find that PRP-1 columns last significantly longer than silica-based C8 or C18 columns. This is particularly true for difficult analyses such as high pH samples (pH 8 to 13), labile or reactive samples (irreversible adsorption), high aqueous purifications (80 to 100% water), and separations with ion pairing reagents.

Stability from pH 1 to 13 allows for the analysis of samples at high pH (8 to 13) without the possibility of column degradation or loss of efficiency. An expanded pH range also allows samples to be chromatographed while ionized.

Sample Recovery is vital to sample purification efforts. Problems arise when labile sample become irreversibly attached to the silanol groups present on C8 and C18 columns. Since PRP-1 is made entirely of polystyrene-divinylbenzene, there are no silanol groups to cause sample loss. Thus, the recovery and quantitation of labile and reactive samples is enhanced.

PRP-1 columns are packed with a poly(styrene-divinylbenzene) material. This support is pressure stable up to 5000 psi, and cross-linked to prevent shrinkage or swelling (common in other polymeric columns). Since the support is entirely polymeric with no silica to deteriorate, column lifetime is longer for PRP-1 than silica C4, C8 or C18 columns.

Housing Material: Anodized Aluminum
Connecting Tubing Size/Material: ¹/₁₆" Out.Ø, 0.28 mm Int.Ø/316 Stainless Steel
Cartridge Materials,
Barrel: CTFE
Frits: 316 Stainless Steel
Sleeve: 304 Stainless Steel
Frit Porosity: 2 µm
Cartridge Dimension: 20×2.3 mm Int.Ø
Cartridge Bed Volume: 80 µl
Particle Size: 12 to 20 µm for Polymeric packings, 10 µm for Silica supports
Chemical Compatibility: Recommended for use with virtually all solvents except high concentrations of sodium chloride.

Ordering information: For PRP-1 Analytical Guard Column Cartridge Kits, see series 21493-100 (stainless steel) and 21493-433 (PEEK). For PRP-1 Analytical Guard Column Replacement Cartridges, see series 21493-225.
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