Protein Purification and Thiophilic Adsorption of IgG, IgM and IgY, G-Biosciences

Supplier: G-Biosciences

786-266 786-267 786-268
CA71003-096EA 1967.08 CAD
CA71003-096 CA71003-098 CA71003-100
Protein Purification and Thiophilic Adsorption of IgG, IgM and IgY, G-Biosciences
Protein Purification Protein Purification Resins

G-Biosciences' Thiophilic Resin is designed for the thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins


G-Biosciences' Thiophilic Resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer. The thiophilic resin has a high binding capacity (~20 mg/mL human IgG/mL resin) and a broad specificity for various species’ immunoglobulin molecules. Thiophilic adsorption has been used to purify other proteins including horseradish peroxidase, glutathione peroxidase, lactate dehydrogenase and allergens.


G-Biosciences' Thiophilic Resin is supplied with protocols for IgG purification, IgM purification, IgY purification and general protein purification. The Thiophilic Adsorption kit is supplied with the thiophilic resin and all the necessary buffers for the rapid purification of immunoglobulin G (IgG) antibodies.


Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2-macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilized ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations.


Ordering information: Accessories included. Additional supplies available.

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