Sulfhydryl Coupling Resin for Antibody Purification, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Sulfhydryl Coupling Resin is designed for the simple and efficient coupling of peptides and proteins to a solid 6% agarose support through free sulfhydryl groups (-SH)
G-Biosciences' Sulfhydryl Immobilization Kit for Proteins is a complete kit designed for the simple and efficient coupling of proteins to a solid agarose support. Proteins, including antibodies, must have free sulfhydryls for immobilization to the resin. A mild reducing agent, 2-Mercaptoethylamine, is supplied to reduce the hinge region disulfide bonds of antibodies, while preserving the functionally crucial disulfide bonds between the heavy and light chains. The resulting columns can be used to study protein-protein interactions or for purification, via affinity chromatography. The columns, depending on the stability of the immobilized molecule, can be used several times without significant loss of activity.
G-Biosciences' Sulfhydryl Immobilization Kit for Peptides is designed for the simple and efficient coupling of sulfhydryl-containing peptides to a solid agarose support. Peptides must have free sulfhydryls for immobilization to the resin. The supplied Protein-S-S-Reductant™ reducing agent efficiently reduces disulfide bonds and does not interfere with the iodoacetyl coupling reaction. Protein-S-S-Reductant™ offers the advantage that it does not require removal before peptide immobilization. The resulting columns can be used for the purification of antibodies that have been raised against the specific peptide. The columns, depending on peptide stability, can be used several times.
The iodoacetyl groups of the Sulfhydryl Coupling Resin specifically react with free sulfhydryls to form covalent, permanent thioether bonds. The long spacer arm reduces steric hindrance and ensures greater binding of proteins and antibodies during affinity purification. The Sulfhydryl Coupling Resin is available as a resin slurry or prealiquoted as five 2ml spin column format.
Ordering information: Accessories included. Additional supplies available.
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