GET™ Plasmid DNA for Plasmid DNA Isolation, G-Biosciences

Supplier: G-Biosciences
GET™
786-648 786-361 786-362 786-648 786-362 786-361
CA78000-054PK 432.35 CAD
CA78000-054 78000-056 78000-054 CA89130-996 CA78000-056 89130-996
GET™ Plasmid DNA for Plasmid DNA Isolation, G-Biosciences
Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents
G-Biosciences' GET™ Plasmid Miniprep kit isolates high quality plasmid DNA from 1-5 mL E

G-Biosciences' GET™ Plasmid DNA Mediprep isolates super-coiled plasmid DNA from 25-200 mL E. coli cultures. The extraction method is based on alkaline lysis of bacteria and uses our high affinity GET™ Plasmid Medi Columns to bind and purify plasmid DNA. The extracted plasmid is ready for further DNA manipulation, such as restriction enzyme digestion, ligation, transformation, transfection and sequencing. The yield is on average 20-100 µg per prep depending on the volume of bacteria and plasmid copy number.

G-Biosciences' GET™ Plasmid DNA Maxiprep isolates super-coiled plasmid DNA from 100-500 mL E. coli cultures. The extraction method is based on alkaline lysis of bacteria and uses our high affinity GET™ Plasmid Maxi Columns to bind and purify plasmid DNA. The extracted plasmid is ready for further DNA manipulation, such as restriction enzyme digestion, ligation, transformation, transfection and sequencing. The yield is on average 100-500 µg per prep depending on the volume of bacteria and plasmid copy number.

G-Biosciences' GET™ Plasmid DNA 96-Well kit is designed for the high thoroughput preparation of plasmid DNA from 96 x 1-5 mL E. coli cultures. The kit utilizes a 96-well format enhanced DNA binding plate to produce high yields of plasmid. This quick and easy protocol eliminates toxic phenol/chloroform extractions or lengthy ethanol precipitations. On completion of the protocol, the plasmid DNA is ready for restriction enzyme digestion, sequencing, subcloning and in vitro transcription. The plasmid yields are typically up to 20 µg per prep.

coli cultures. The kit utilizes an enhanced DNA binding column to produce high yields of plasmid. This quick and easy protocol eliminates toxic phenol/chloroform extractions or lengthy ethanol precipitations. On completion of the protocol, the plasmid DNA is ready for restriction enzyme digestion, sequencing, subcloning and in vitro transcription. The plasmid yields are typically up to 20 μg per prep.

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