Antibody Fab Fragmentation, G-Biosciences

Supplier: G-Biosciences

Antibody Fab Fragmentation, G-Biosciences

Antibody Fab Fragmentation, G-Biosciences

786-272 786-273

CA71003-106EA 2157.76 CAD

CA71003-106 CA71003-108

Antibody Fab Fragmentation, G-Biosciences

Protein Extraction Protein Extraction Kits

G-Biosciences' Fab Fragmentation kits are designed for the generation and isolation of Fab fragments from IgG molecules

Papain is a cysteine protease enzyme that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason. This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonization, and lysis. Immobilized Papain offers the advantage of generating Fab and Fc fragments without the need to remove the papain enzyme after digestion.

Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. The Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected due to the spin-format design.

In addition, SpinOUT™ GT-600 desalting columns are supplied to ensure the initial antibody sample is in the optimal condition for Fab Fragmentation. The Fab Fragmentation kit is optimized for mouse, rabbit and human IgG, using 0.25-4 mg/ 0.5 mL sample. The Fab Fragmentation (Micro) kit is optimized for mouse, rabbit and human IgG, using 25-250 µg/ 125 µL sample.

The kits utilize our Immobilized Papain resin.

Ordering information: Accessories available.

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