G-Biosciences' Well-Coated™ Neutravidin™ plates are designed to specifically bind biotinylated molecules, including biotin tagged antibodies, with minimal non-specific binding
Biotin exhibits an extraordinary binding affinity for avidin (Ka=1015M-1) and Neutravidin™ (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by autoclaving. Neutravidin™ is in many respects is similar to avidin except that it has no carbohydrate side chains to eliminate lectin binding; is of near neutral pI (6.3) to reduce non-specific adsorption; lacks the RYD sequence eliminating interaction with RGD domain of adhesion receptors. The binding of Neutravidin™ is similar to that of avidin and streptavidin with less non-specific binding.
G-Biosciences' Well-Coated™ Neutravidin™ plates are suitable for direct, indirect, competitive and sandwich assays. The wells are coated to a 100µL depth and are supplied pre-blocked in our proprietary Superior™ Blocking Buffer. The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively.
This is particular advantageous for antibodies known to denature upon direct binding to polystyrene plates
Ordering information: Accessories available.
