Well-Coated™ Streptavidin for Biotin Binding, G-Biosciences

Supplier: G-Biosciences
786-745 786-778 786-779
CA89167-962EA 918.22 CAD
CA89167-962 CA89168-028 CA89168-030
Well-Coated™ Streptavidin for Biotin Binding, G-Biosciences
Microplates HTS Microplates
G-Biosciences' Well-Coated™ Streptavidin plates are designed to specifically bind biotinylated molecules, including biotin tagged antibodies

Biotin exhibits an extraordinary binding affinity for avidin (Ka=1015M-1) and streptavidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by autoclaving. Streptavidin is in many respects is similar to avidin except that it has no carbohydrate and the solubility of streptavidin (isoelectric pH5) in aqueous buffer is much lower than avidin. The binding of streptavidin is similar to that of avidin with less non-specific binding due to the lack of carbohydrate groups.

G-Biosciences' Well-Coated™ Streptavidin plates are suitable for direct, indirect, competitive and sandwich assays. The wells are coated to a 100µL depth and are supplied pre-blocked in our proprietary Superior™ Blocking Buffer. The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively.

This is particular advantageous for antibodies known to denature upon direct binding to polystyrene plates.

Ordering information: Accessories available.
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